Gene description for ASAP3
Gene name ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Gene symbol ASAP3
Other names/aliases ACAP4
CENTB6
DDEFL1
UPLC1
Species Homo sapiens
 Database cross references - ASAP3
ExoCarta ExoCarta_55616
Vesiclepedia VP_55616
Entrez Gene 55616
HGNC 14987
UniProt Q8TDY4  
 ASAP3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ASAP3
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    cell migration GO:0016477 IMP
    positive regulation of GTPase activity GO:0043547 IDA
    regulation of stress fiber assembly GO:0051492 IBA
    regulation of stress fiber assembly GO:0051492 IMP
Subcellular Localization
    ruffle GO:0001726 IBA
    ruffle GO:0001726 IDA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 IDA
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified ASAP3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ASAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ELP2 55250
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 KIAA1161 57462
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PSMD14 10213
Affinity Capture-MS Homo sapiens
4 REL 5966
Two-hybrid Homo sapiens
5 GOLGA2 2801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ACAD11 84129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 IKBKG 8517
Affinity Capture-Western Homo sapiens
8 TFCP2 7024
Two-hybrid Homo sapiens
9 RAB11FIP3  
Affinity Capture-Western Homo sapiens
10 UBE3A 7337
Two-hybrid Homo sapiens
11 ASAP1 50807
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MTNR1A  
Two-hybrid Homo sapiens
13 BACH1 571
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PDZD3  
Two-hybrid Homo sapiens
15 CRKL 1399
Affinity Capture-MS Homo sapiens
16 CRK 1398
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 XRCC5 7520
Affinity Capture-MS Homo sapiens
18 TULP3 7289
Affinity Capture-MS Homo sapiens
19 ASAP2 8853
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HSPA1A 3303
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ASAP3 is involved
No pathways found





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