Gene description for NHP2
Gene name NHP2 ribonucleoprotein
Gene symbol NHP2
Other names/aliases DKCB2
NHP2P
NOLA2
Species Homo sapiens
 Database cross references - NHP2
ExoCarta ExoCarta_55651
Vesiclepedia VP_55651
Entrez Gene 55651
HGNC 14377
MIM 606470
UniProt Q9NX24  
 NHP2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NHP2
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IPI
    mRNA 3'-UTR binding GO:0003730 IEA
    protein binding GO:0005515 IPI
    U3 snoRNA binding GO:0034511 IEA
    box H/ACA snoRNA binding GO:0034513 IBA
    box H/ACA snoRNA binding GO:0034513 IPI
    telomerase RNA binding GO:0070034 IPI
Biological Process
    snoRNA guided rRNA pseudouridine synthesis GO:0000454 IEA
    telomere maintenance via telomerase GO:0007004 IDA
    telomere maintenance via telomerase GO:0007004 NAS
    telomere maintenance via telomerase GO:0007004 TAS
    rRNA pseudouridine synthesis GO:0031118 IBA
    rRNA pseudouridine synthesis GO:0031118 ISS
    snRNA pseudouridine synthesis GO:0031120 IBA
    telomerase RNA localization to Cajal body GO:0090671 HMP
Subcellular Localization
    chromosome, telomeric region GO:0000781 HDA
    nucleoplasm GO:0005654 TAS
    telomerase holoenzyme complex GO:0005697 IDA
    telomerase holoenzyme complex GO:0005697 NAS
    telomerase holoenzyme complex GO:0005697 TAS
    sno(s)RNA-containing ribonucleoprotein complex GO:0005732 ISS
    box H/ACA snoRNP complex GO:0031429 IBA
    box H/ACA snoRNP complex GO:0031429 IDA
    box H/ACA snoRNP complex GO:0031429 TAS
    box H/ACA scaRNP complex GO:0072589 TAS
    box H/ACA telomerase RNP complex GO:0090661 IDA
    box H/ACA telomerase RNP complex GO:0090661 TAS
 Experiment description of studies that identified NHP2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NHP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 NOP58 51602
Co-fractionation Homo sapiens
5 FBXO33  
Affinity Capture-MS Homo sapiens
6 MRPL30  
Affinity Capture-MS Homo sapiens
7 MRPL17  
Affinity Capture-MS Homo sapiens
8 BTF3 689
Affinity Capture-MS Homo sapiens
9 SUB1 10923
Affinity Capture-MS Homo sapiens
10 KIF20A 10112
Affinity Capture-MS Homo sapiens
11 MRPL27 51264
Affinity Capture-MS Homo sapiens
12 SSB 6741
Affinity Capture-MS Homo sapiens
13 MRPL50 54534
Affinity Capture-MS Homo sapiens
14 CAND1 55832
Affinity Capture-MS Homo sapiens
15 MRPL49 740
Affinity Capture-MS Homo sapiens
16 SOX2  
Affinity Capture-MS Homo sapiens
17 USP36  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
18 WDR48 57599
Affinity Capture-MS Homo sapiens
19 Nedd4 25489
Reconstituted Complex Rattus norvegicus
20 NOLC1 9221
Affinity Capture-Western Homo sapiens
21 FBL 2091
Co-fractionation Homo sapiens
22 SNORA66  
Affinity Capture-RNA Homo sapiens
23 EIF5B 9669
Co-fractionation Homo sapiens
24 RSRC1  
Affinity Capture-MS Homo sapiens
25 SNORA24  
Affinity Capture-RNA Homo sapiens
26 MRPL39 54148
Affinity Capture-MS Homo sapiens
27 ATG16L1 55054
Affinity Capture-MS Homo sapiens
28 MYCN  
Affinity Capture-MS Homo sapiens
29 MRPL4 51073
Affinity Capture-MS Homo sapiens
30 NEDD4 4734
Reconstituted Complex Homo sapiens
31 GAR1 54433
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PRNP 5621
Reconstituted Complex Homo sapiens
33 LIN28A  
Affinity Capture-MS Homo sapiens
34 CHMP4C 92421
Affinity Capture-MS Homo sapiens
35 TCOF1 6949
Affinity Capture-Western Homo sapiens
36 JMJD6 23210
Two-hybrid Homo sapiens
37 COX15 1355
Affinity Capture-MS Homo sapiens
38 DDX21 9188
Affinity Capture-MS Homo sapiens
39 LIN28B  
Affinity Capture-MS Homo sapiens
40 WHSC1 7468
Proximity Label-MS Homo sapiens
41 SRSF6 6431
Affinity Capture-MS Homo sapiens
42 UBE2I 7329
Biochemical Activity Homo sapiens
43 SNORA75 654321
Affinity Capture-RNA Homo sapiens
44 NEDD4L 23327
Reconstituted Complex Homo sapiens
45 FOXK2  
Affinity Capture-MS Homo sapiens
46 BCHE 590
Affinity Capture-MS Homo sapiens
47 ZNF131  
Affinity Capture-MS Homo sapiens
48 LYAR 55646
Affinity Capture-MS Homo sapiens
49 Nhp2l1 20826
Affinity Capture-MS Mus musculus
50 Ube2i  
Two-hybrid Mus musculus
51 TERC  
Affinity Capture-Western Homo sapiens
52 DAXX  
Affinity Capture-MS Homo sapiens
53 ZBTB2 57621
Affinity Capture-MS Homo sapiens
54 MRPL21 219927
Affinity Capture-MS Homo sapiens
55 SNORA10  
Affinity Capture-RNA Homo sapiens
56 XPO1 7514
Affinity Capture-MS Homo sapiens
57 RC3H2  
Affinity Capture-MS Homo sapiens
58 IKZF3  
Affinity Capture-MS Homo sapiens
59 NR3C1 2908
Proximity Label-MS Homo sapiens
60 SRSF5 6430
Affinity Capture-MS Homo sapiens
61 IKZF1  
Affinity Capture-MS Homo sapiens
62 NAF1  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
63 SUZ12  
Affinity Capture-MS Homo sapiens
64 FN1 2335
Affinity Capture-MS Homo sapiens
65 MRPL19 9801
Affinity Capture-MS Homo sapiens
66 DKC1 1736
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
67 MYC  
Affinity Capture-MS Homo sapiens
68 NOL12 79159
Affinity Capture-MS Homo sapiens
69 DLST 1743
Affinity Capture-MS Homo sapiens
70 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
71 MYO9A 4649
Affinity Capture-MS Homo sapiens
72 SRSF10 10772
Affinity Capture-MS Homo sapiens
73 RNF2  
Affinity Capture-MS Homo sapiens
74 BMI1  
Affinity Capture-MS Homo sapiens
75 ARGLU1  
Affinity Capture-MS Homo sapiens
76 MRPL38  
Affinity Capture-MS Homo sapiens
77 MRPL18 29074
Affinity Capture-MS Homo sapiens
78 HUWE1 10075
Affinity Capture-MS Homo sapiens
79 YBX1 4904
Affinity Capture-MS Homo sapiens
80 CIT 11113
Affinity Capture-MS Homo sapiens
81 MNDA 4332
Affinity Capture-MS Homo sapiens
82 SNORA64  
Affinity Capture-RNA Homo sapiens
83 ARHGEF10L 55160
Affinity Capture-MS Homo sapiens
84 IKBKG 8517
Reconstituted Complex Homo sapiens
85 UTP23  
Reconstituted Complex Homo sapiens
86 CWF19L2  
Affinity Capture-MS Homo sapiens
87 TRA2B 6434
Affinity Capture-MS Homo sapiens
88 MYL2  
Two-hybrid Homo sapiens
89 SSBP1 6742
Affinity Capture-MS Homo sapiens
90 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
91 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
92 PURA 5813
Affinity Capture-MS Homo sapiens
93 NOP10 55505
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
94 Srrm3 58212
Two-hybrid Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here