Gene description for PRKCE
Gene name protein kinase C, epsilon
Gene symbol PRKCE
Other names/aliases PKCE
nPKC-epsilon
Species Homo sapiens
 Database cross references - PRKCE
ExoCarta ExoCarta_5581
Vesiclepedia VP_5581
Entrez Gene 5581
HGNC 9401
MIM 176975
UniProt Q02156  
 PRKCE identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PRKCE
Molecular Function
    actin monomer binding GO:0003785 ISS
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 TAS
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IEA
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme activator activity GO:0008047 IMP
    enzyme binding GO:0019899 IDA
    enzyme binding GO:0019899 IPI
    signaling receptor activator activity GO:0030546 IEA
    ethanol binding GO:0035276 IEA
    metal ion binding GO:0046872 IEA
    14-3-3 protein binding GO:0071889 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    MAPK cascade GO:0000165 IEA
    macrophage activation involved in immune response GO:0002281 IEA
    protein phosphorylation GO:0006468 IDA
    apoptotic process GO:0006915 TAS
    signal transduction GO:0007165 TAS
    toxin catabolic process GO:0009407 IDA
    positive regulation of epithelial cell migration GO:0010634 IMP
    positive regulation of fibroblast migration GO:0010763 ISS
    positive regulation of cell-substrate adhesion GO:0010811 IEA
    peptidyl-serine phosphorylation GO:0018105 IDA
    insulin secretion GO:0030073 IEA
    positive regulation of actin filament polymerization GO:0030838 ISS
    negative regulation of protein ubiquitination GO:0031397 IEA
    cell-substrate adhesion GO:0031589 IEA
    lipopolysaccharide-mediated signaling pathway GO:0031663 ISS
    positive regulation of insulin secretion GO:0032024 IEA
    positive regulation of synaptic transmission, GABAergic GO:0032230 IEA
    positive regulation of cytokinesis GO:0032467 IMP
    positive regulation of superoxide anion generation GO:0032930 TAS
    intracellular signal transduction GO:0035556 IBA
    locomotory exploration behavior GO:0035641 IEA
    TRAM-dependent toll-like receptor 4 signaling pathway GO:0035669 ISS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEA
    response to morphine GO:0043278 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of lipid catabolic process GO:0050996 IEA
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IEA
    cell division GO:0051301 IEA
    establishment of localization in cell GO:0051649 IEA
    synaptic transmission, GABAergic GO:0051932 IEA
    regulation of insulin secretion involved in cellular response to glucose stimulus GO:0061178 IEA
    mucus secretion GO:0070254 IEA
    positive regulation of mucus secretion GO:0070257 IEA
    cellular response to ethanol GO:0071361 IEA
    cellular response to prostaglandin E stimulus GO:0071380 IEA
    cellular response to hypoxia GO:0071456 IEA
    positive regulation of wound healing GO:0090303 IMP
    negative regulation of sodium ion transmembrane transport GO:1902306 TAS
    positive regulation of protein localization to plasma membrane GO:1903078 TAS
    positive regulation of cellular glucuronidation GO:2001031 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IEA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    intermediate filament cytoskeleton GO:0045111 IDA
    synapse GO:0045202 IEA
    perinuclear region of cytoplasm GO:0048471 ISS
    cell periphery GO:0071944 ISS
 Experiment description of studies that identified PRKCE in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRKCE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HK2 3099
Affinity Capture-Western Homo sapiens
2 KRT18 3875
Affinity Capture-Western Homo sapiens
3 PCBP3 54039
Affinity Capture-MS Homo sapiens
4 GNA12 2768
Biochemical Activity Homo sapiens
5 MAPT  
Biochemical Activity Homo sapiens
6 GAD2  
Biochemical Activity Homo sapiens
7 RASGRP3  
Biochemical Activity Homo sapiens
8 BAD  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 OGFR 11054
Affinity Capture-MS Homo sapiens
10 GJA1 2697
Affinity Capture-Western Homo sapiens
11 MAPK1 5594
Affinity Capture-Western Homo sapiens
12 NANOG  
Biochemical Activity Homo sapiens
13 IL32  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 KRT8 3856
Affinity Capture-Western Homo sapiens
15 ADAP1  
Biochemical Activity Homo sapiens
16 AMY1C 278
Affinity Capture-MS Homo sapiens
17 PRKCH 5583
Affinity Capture-MS Homo sapiens
18 KCNC4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 GAD1  
Biochemical Activity Homo sapiens
20 IKBKG 8517
Affinity Capture-Western Homo sapiens
21 HSP90AB1 3326
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 YWHAZ 7534
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 TIMM10B 26515
Affinity Capture-MS Homo sapiens
24 MAP4K4 9448
Two-hybrid Homo sapiens
25 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
Negative Genetic Homo sapiens
26 ELAVL1 1994
Biochemical Activity Homo sapiens
27 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
28 ZBTB16  
Affinity Capture-Western Homo sapiens
29 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
30 PAPOLG  
Affinity Capture-MS Homo sapiens
31 BPIFA2 140683
Affinity Capture-MS Homo sapiens
32 RPS6KB2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 Marcks 17118
Biochemical Activity Mus musculus
34 CST4 1472
Affinity Capture-MS Homo sapiens
35 ATF2  
Biochemical Activity Homo sapiens
36 CST2  
Affinity Capture-MS Homo sapiens
37 PPP1R14A 94274
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
38 ACTA1 58
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
39 PDLIM5 10611
Two-hybrid Homo sapiens
40 MARK2 2011
Biochemical Activity Homo sapiens
41 AFAP1 60312
Reconstituted Complex Homo sapiens
42 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
43 SLC25A4 291
Affinity Capture-Western Homo sapiens
44 MYH9 4627
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
45 PIK3CB 5291
Affinity Capture-Western Homo sapiens
46 Prkcz  
Biochemical Activity Rattus norvegicus
47 IBTK 25998
Affinity Capture-Western Homo sapiens
48 ZG16B 124220
Affinity Capture-MS Homo sapiens
49 COPB2 9276
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
50 KAT5  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
51 HIST1H1A 3024
Biochemical Activity Homo sapiens
52 RAF1 5894
Affinity Capture-Western Homo sapiens
53 GNA13 10672
Biochemical Activity Homo sapiens
54 BCL6  
Affinity Capture-Western Homo sapiens
55 GNB2L1 10399
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
56 VDAC1 7416
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
57 BRAF  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 CFTR 1080
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 DDX58 23586
Affinity Capture-RNA Homo sapiens
60 TIAM1  
Biochemical Activity Homo sapiens
61 ITGB2 3689
Biochemical Activity Homo sapiens
62 IKBKB 3551
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
63 KRT1 3848
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
64 CDC37 11140
Affinity Capture-MS Homo sapiens
65 DSP 1832
Two-hybrid Homo sapiens
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