Gene description for PRKCG
Gene name protein kinase C, gamma
Gene symbol PRKCG
Other names/aliases PKC-gamma
PKCC
PKCG
SCA14
Species Homo sapiens
 Database cross references - PRKCG
ExoCarta ExoCarta_5582
Vesiclepedia VP_5582
Entrez Gene 5582
HGNC 9402
MIM 176980
UniProt P05129  
 PRKCG identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for PRKCG
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 TAS
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IDA
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 IEA
    protein serine/threonine/tyrosine kinase activity GO:0004712 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    protein phosphorylation GO:0006468 TAS
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IDA
    chemical synaptic transmission GO:0007268 IEA
    learning or memory GO:0007611 IEA
    chemosensory behavior GO:0007635 IEA
    response to toxic substance GO:0009636 IEA
    phosphorylation GO:0016310 IDA
    negative regulation of protein ubiquitination GO:0031397 IDA
    regulation of response to food GO:0032095 ISS
    positive regulation of mismatch repair GO:0032425 IDA
    intracellular signal transduction GO:0035556 IBA
    negative regulation of protein catabolic process GO:0042177 IDA
    regulation of circadian rhythm GO:0042752 ISS
    response to morphine GO:0043278 ISS
    negative regulation of neuron apoptotic process GO:0043524 ISS
    response to pain GO:0048265 ISS
    rhythmic process GO:0048511 IEA
    regulation of phagocytosis GO:0050764 IEA
    long-term synaptic potentiation GO:0060291 IEA
    innervation GO:0060384 IEA
    presynaptic modulation of chemical synaptic transmission GO:0099171 IEA
    negative regulation of proteasomal protein catabolic process GO:1901799 ISS
    response to psychosocial stress GO:1990911 IEA
    regulation of synaptic vesicle exocytosis GO:2000300 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISS
    cell-cell junction GO:0005911 IEA
    postsynaptic density GO:0014069 IEA
    dendrite GO:0030425 ISS
    calyx of Held GO:0044305 IEA
    perinuclear region of cytoplasm GO:0048471 ISS
    synaptic membrane GO:0097060 IEA
    presynaptic cytosol GO:0099523 IEA
    postsynaptic cytosol GO:0099524 TAS
 Experiment description of studies that identified PRKCG in sEVs
1
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for PRKCG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SDC2 6383
Biochemical Activity Homo sapiens
2 TRIM5 85363
Reconstituted Complex Homo sapiens
3 ARHGEF25 115557
Reconstituted Complex Homo sapiens
4 PICK1  
Two-hybrid Homo sapiens
5 EXOC5 10640
Two-hybrid Homo sapiens
6 RANBP10 57610
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 RGS2  
Biochemical Activity Homo sapiens
8 CCHCR1  
Two-hybrid Homo sapiens
9 SCN3A  
Reconstituted Complex Homo sapiens
10 Marcks 17118
Biochemical Activity Mus musculus
11 CTNNB1 1499
Biochemical Activity Homo sapiens
12 GRIA4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 GFAP 2670
Reconstituted Complex Homo sapiens
14 SMURF1 57154
Biochemical Activity Homo sapiens
15 EPHB1 2047
Reconstituted Complex Homo sapiens
16 UBE2T  
Biochemical Activity Homo sapiens
17 EGFR 1956
Negative Genetic Homo sapiens
18 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
19 DDX58 23586
Biochemical Activity Homo sapiens
20 APP 351
Reconstituted Complex Homo sapiens
21 GABRA1  
Affinity Capture-Western Homo sapiens
22 GSK3A 2931
Biochemical Activity Homo sapiens
23 RANBP9 10048
Affinity Capture-Western Homo sapiens
24 TOP2A 7153
Biochemical Activity Homo sapiens
25 AFAP1 60312
Reconstituted Complex Homo sapiens
26 MAPT  
Biochemical Activity Homo sapiens
27 IBTK 25998
Affinity Capture-Western Homo sapiens
28 PRKCA 5578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 HSPA4 3308
Reconstituted Complex Homo sapiens
30 ITGB2 3689
Biochemical Activity Homo sapiens
31 NOXA1  
Two-hybrid Homo sapiens
32 YWHAG 7532
Affinity Capture-Western Homo sapiens
33 TIAM1  
Biochemical Activity Homo sapiens
34 GABRA4  
Affinity Capture-Western Homo sapiens
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