Gene description for WDR45B
Gene name WD repeat domain 45B
Gene symbol WDR45B
Other names/aliases WDR45L
WIPI-3
WIPI3
Species Homo sapiens
 Database cross references - WDR45B
ExoCarta ExoCarta_56270
Vesiclepedia VP_56270
Entrez Gene 56270
HGNC 25072
MIM 609226
UniProt Q5MNZ6  
 WDR45B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WDR45B
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-3-phosphate binding GO:0032266 IBA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    TSC1-TSC2 complex binding GO:0062078 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IBA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
Biological Process
    autophagosome assembly GO:0000045 IMP
    autophagy of mitochondrion GO:0000422 IBA
    cellular response to starvation GO:0009267 IDA
    autophagy of peroxisome GO:0030242 IBA
    protein localization to phagophore assembly site GO:0034497 IBA
    nucleophagy GO:0044804 IBA
    glycophagy GO:0061723 IBA
    protein lipidation involved in autophagosome assembly GO:0061739 IBA
Subcellular Localization
    phagophore assembly site GO:0000407 IDA
    lysosome GO:0005764 IDA
    cytosol GO:0005829 IBA
    phagophore assembly site membrane GO:0034045 IBA
 Experiment description of studies that identified WDR45B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WDR45B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCY  
Affinity Capture-MS Homo sapiens
2 AK4 205
Affinity Capture-MS Homo sapiens
3 EIF1AD 84285
Affinity Capture-MS Homo sapiens
4 SNX27 81609
Affinity Capture-MS Homo sapiens
5 LAIR2  
Affinity Capture-MS Homo sapiens
6 AHDC1  
Affinity Capture-MS Homo sapiens
7 SPX  
Affinity Capture-MS Homo sapiens
8 SCYL3 57147
Affinity Capture-MS Homo sapiens
9 RNF181  
Affinity Capture-MS Homo sapiens
10 C5orf24 134553
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CACNB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 UBXN10  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 CCL15  
Affinity Capture-MS Homo sapiens
14 MID1IP1  
Affinity Capture-MS Homo sapiens
15 POLE3  
Affinity Capture-MS Homo sapiens
16 TNFRSF10D 8793
Affinity Capture-MS Homo sapiens
17 CNPY2 10330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TFAP4 7023
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TYMS 7298
Co-fractionation Homo sapiens
20 C17orf49 124944
Affinity Capture-MS Homo sapiens
21 ARRDC1 92714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
23 PLEKHA1 59338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SPA17  
Affinity Capture-MS Homo sapiens
25 PLEKHO1 51177
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 LSS 4047
Affinity Capture-MS Homo sapiens
27 TXNDC17 84817
Co-fractionation Homo sapiens
28 C19orf25  
Affinity Capture-MS Homo sapiens
29 TMEM263 90488
Affinity Capture-MS Homo sapiens
30 CUX2 23316
Affinity Capture-MS Homo sapiens
31 ASPRV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MTMR9 66036
Affinity Capture-MS Homo sapiens
33 PMF1  
Affinity Capture-MS Homo sapiens
34 ILVBL 10994
Affinity Capture-MS Homo sapiens
35 AKR1B1 231
Affinity Capture-MS Homo sapiens
36 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
37 DUSP13  
Affinity Capture-MS Homo sapiens
38 KHDRBS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
40 MT1F  
Affinity Capture-MS Homo sapiens
41 RPA3 6119
Proximity Label-MS Homo sapiens
42 ANKRD39  
Affinity Capture-MS Homo sapiens
43 PMF1-BGLAP  
Affinity Capture-MS Homo sapiens
44 PLEKHN1  
Affinity Capture-MS Homo sapiens
45 SLC9A3R1 9368
Affinity Capture-MS Homo sapiens
46 SARNP 84324
Affinity Capture-MS Homo sapiens
47 P2RX5 5026
Affinity Capture-MS Homo sapiens
48 EVA1B 55194
Affinity Capture-MS Homo sapiens
49 PML 5371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 RAB3A 5864
Affinity Capture-MS Homo sapiens
51 GAS2L1 10634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 BMX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 KCNJ5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 APP 351
Reconstituted Complex Homo sapiens
55 RCN1 5954
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 UPP1 7378
Co-fractionation Homo sapiens
57 UROD 7389
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which WDR45B is involved
PathwayEvidenceSource
Autophagy TAS Reactome
Macroautophagy TAS Reactome





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