Experiment description of studies that identified PRRG1 in sEVs

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for PRRG1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 STXBP4 252983 Affinity Capture-MS Homo sapiens 2 DCAF10   Affinity Capture-MS Homo sapiens 3 GGCX 2677 Affinity Capture-MS Homo sapiens 4 FBXO25   Biochemical Activity Homo sapiens 5 HECW2   Affinity Capture-MS Homo sapiens 6 UQCRH 7388 Two-hybrid Homo sapiens 7 NEDD4 4734 Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 ITCH 83737 Affinity Capture-MS Homo sapiens 9 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 10 HECW1   Affinity Capture-MS Homo sapiens 11 Nedd4 25489 Reconstituted Complex Rattus norvegicus 12 NEDD4L 23327 Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens 13 WWP1 11059 Affinity Capture-MS Homo sapiens 14 FBXO10   Affinity Capture-MS Homo sapiens 15 YAP1 10413 Affinity Capture-MS Homo sapiens View the network

Pathways in which PRRG1 is involved

No pathways found