Gene description for RELN
Gene name reelin
Gene symbol RELN
Other names/aliases LIS2
PRO1598
RL
Species Homo sapiens
 Database cross references - RELN
ExoCarta ExoCarta_5649
Vesiclepedia VP_5649
Entrez Gene 5649
HGNC 9957
MIM 600514
UniProt P78509  
 RELN identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Plasma 19028452    
 Gene ontology annotations for RELN
Molecular Function
    serine-type peptidase activity GO:0008236 ISS
    metal ion binding GO:0046872 IEA
    receptor ligand activity GO:0048018 ISS
    lipoprotein particle receptor binding GO:0070325 IBA
    lipoprotein particle receptor binding GO:0070325 ISS
    very-low-density lipoprotein particle receptor binding GO:0070326 ISS
Biological Process
    cell morphogenesis GO:0000902 ISS
    neuron migration GO:0001764 IBA
    neuron migration GO:0001764 ISS
    proteolysis GO:0006508 IEA
    cell adhesion GO:0007155 IEA
    axon guidance GO:0007411 ISS
    central nervous system development GO:0007417 IBA
    central nervous system development GO:0007417 ISS
    brain development GO:0007420 ISS
    long-term memory GO:0007616 ISS
    locomotory behavior GO:0007626 IEA
    associative learning GO:0008306 ISS
    glial cell differentiation GO:0010001 ISS
    regulation of gene expression GO:0010468 IEA
    positive regulation of neuron projection development GO:0010976 ISS
    dendrite development GO:0016358 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 ISS
    spinal cord patterning GO:0021511 ISS
    ventral spinal cord development GO:0021517 IEA
    hippocampus development GO:0021766 ISS
    cerebral cortex tangential migration GO:0021800 ISS
    layer formation in cerebral cortex GO:0021819 ISS
    positive regulation of TOR signaling GO:0032008 ISS
    protein localization to synapse GO:0035418 ISS
    reelin-mediated signaling pathway GO:0038026 ISS
    reelin-mediated signaling pathway GO:0038026 NAS
    regulation of neuron differentiation GO:0045664 NAS
    positive regulation of protein kinase activity GO:0045860 ISS
    response to pain GO:0048265 ISS
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISS
    regulation of behavior GO:0050795 ISS
    modulation of chemical synaptic transmission GO:0050804 ISS
    positive regulation of small GTPase mediated signal transduction GO:0051057 ISS
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISS
    positive regulation of synaptic transmission, glutamatergic GO:0051968 ISS
    regulation of synaptic activity GO:0060025 NAS
    long-term synaptic potentiation GO:0060291 IEA
    positive regulation of dendritic spine morphogenesis GO:0061003 ISS
    positive regulation of synapse maturation GO:0090129 ISS
    postsynaptic density assembly GO:0097107 ISS
    NMDA glutamate receptor clustering GO:0097114 ISS
    postsynaptic density protein 95 clustering GO:0097119 ISS
    receptor localization to synapse GO:0097120 ISS
    lateral motor column neuron migration GO:0097477 IEA
    positive regulation of long-term synaptic potentiation GO:1900273 ISS
    positive regulation of lateral motor column neuron migration GO:1902078 IEA
    interneuron migration GO:1904936 IEA
    regulation of NMDA receptor activity GO:2000310 ISS
    positive regulation of excitatory postsynaptic potential GO:2000463 ISS
    regulation of neuron migration GO:2001222 NAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IEA
    dendrite GO:0030425 ISS
    extracellular matrix GO:0031012 IEA
    neuron projection GO:0043005 IBA
    reelin complex GO:0110157 IDA
 Experiment description of studies that identified RELN in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for RELN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COPS5 10987
Affinity Capture-MS Homo sapiens
2 RELN 5649
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 VLDLR 7436
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 MECP2 4204
Affinity Capture-MS Homo sapiens
5 TET1  
Co-localization Homo sapiens
6 DNMT1 1786
Co-localization Homo sapiens
7 MBD2 8932
Co-localization Homo sapiens
8 LRP8 7804
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which RELN is involved
PathwayEvidenceSource
Axon guidance TAS Reactome
Developmental Biology TAS Reactome
Nervous system development TAS Reactome
Reelin signalling pathway TAS Reactome





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