Gene description for GRHL3
Gene name grainyhead-like 3 (Drosophila)
Gene symbol GRHL3
Other names/aliases SOM
TFCP2L4
VWS2
Species Homo sapiens
 Database cross references - GRHL3
ExoCarta ExoCarta_57822
Vesiclepedia VP_57822
Entrez Gene 57822
HGNC 25839
MIM 608317
UniProt Q8TE85  
 GRHL3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GRHL3
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IBA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    protein binding GO:0005515 IPI
    chromatin DNA binding GO:0031490 IDA
    sequence-specific DNA binding GO:0043565 ISS
Biological Process
    establishment of planar polarity GO:0001736 IEA
    neural tube closure GO:0001843 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription by RNA polymerase II GO:0006366 IEA
    pattern specification process GO:0007389 IEA
    ectoderm development GO:0007398 IEA
    central nervous system development GO:0007417 IEA
    epidermis development GO:0008544 ISS
    positive regulation of gene expression GO:0010628 IEA
    regulation of actin cytoskeleton organization GO:0032956 IGI
    wound healing GO:0042060 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    eyelid development in camera-type eye GO:0061029 IEA
    establishment of skin barrier GO:0061436 IEA
    cochlea morphogenesis GO:0090103 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified GRHL3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GRHL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIPPLY1  
Two-hybrid Homo sapiens
2 ENKD1  
Two-hybrid Homo sapiens
3 GRHL1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 PRMT6  
Two-hybrid Homo sapiens
5 VSIG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PRMT1 3276
Two-hybrid Homo sapiens
7 PRMT5 10419
Two-hybrid Homo sapiens
8 EAF1  
Two-hybrid Homo sapiens
9 GRHL3 57822
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 GRHL2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which GRHL3 is involved
No pathways found





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