Gene description for SMAP1
Gene name small ArfGAP 1
Gene symbol SMAP1
Other names/aliases SMAP-1
Species Homo sapiens
 Database cross references - SMAP1
ExoCarta ExoCarta_60682
Vesiclepedia VP_60682
Entrez Gene 60682
HGNC 19651
MIM 611372
UniProt Q8IYB5  
 SMAP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMAP1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    protein binding GO:0005515 IPI
    clathrin binding GO:0030276 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    positive regulation of erythrocyte differentiation GO:0045648 IEA
    regulation of clathrin-dependent endocytosis GO:2000369 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified SMAP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRF2  
Affinity Capture-MS Homo sapiens
2 KLHL18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SNX27 81609
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 RBPMS 11030
Two-hybrid Homo sapiens
6 PRR20C  
Two-hybrid Homo sapiens
7 NPW  
Affinity Capture-MS Homo sapiens
8 PRR20A  
Two-hybrid Homo sapiens
9 AIPL1  
Two-hybrid Homo sapiens
10 CRX  
Two-hybrid Homo sapiens
11 RAB5A 5868
Proximity Label-MS Homo sapiens
12 BAG2 9532
Affinity Capture-MS Homo sapiens
13 PHLDA3 23612
Affinity Capture-MS Homo sapiens
14 E2F4  
Affinity Capture-MS Homo sapiens
15 ATG9A 79065
Proximity Label-MS Homo sapiens
16 HSPA1L 3305
Affinity Capture-MS Homo sapiens
17 ARF3 377
Proximity Label-MS Homo sapiens
18 SQSTM1 8878
Proximity Label-MS Homo sapiens
19 PRR20D  
Two-hybrid Homo sapiens
20 ZNF829  
Affinity Capture-MS Homo sapiens
21 CLTB 1212
Proximity Label-MS Homo sapiens
22 APP 351
Reconstituted Complex Homo sapiens
23 WRAP73 49856
Affinity Capture-MS Homo sapiens
24 MICU2 221154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 XPO1 7514
Affinity Capture-MS Homo sapiens
26 PRR20B  
Two-hybrid Homo sapiens
27 PTP4A3  
Affinity Capture-MS Homo sapiens
28 PRR20E  
Two-hybrid Homo sapiens
29 QRICH1  
Two-hybrid Homo sapiens
30 RPA3 6119
Proximity Label-MS Homo sapiens
31 WDYHV1  
Two-hybrid Homo sapiens
32 CLTA 1211
Proximity Label-MS Homo sapiens
33 HSPA1A 3303
Affinity Capture-MS Homo sapiens
34 RPA4  
Proximity Label-MS Homo sapiens
35 PFDN5 5204
Two-hybrid Homo sapiens
36 STX6 10228
Proximity Label-MS Homo sapiens
37 PHGDH 26227
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which SMAP1 is involved
No pathways found





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