Gene description for SCD
Gene name stearoyl-CoA desaturase (delta-9-desaturase)
Gene symbol SCD
Other names/aliases FADS5
MSTP008
SCD1
SCDOS
Species Homo sapiens
 Database cross references - SCD
ExoCarta ExoCarta_6319
Vesiclepedia VP_6319
Entrez Gene 6319
HGNC 10571
MIM 604031
UniProt O00767  
 SCD identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SCD
Molecular Function
    stearoyl-CoA 9-desaturase activity GO:0004768 IBA
    stearoyl-CoA 9-desaturase activity GO:0004768 IDA
    iron ion binding GO:0005506 IBA
    iron ion binding GO:0005506 IDA
    protein binding GO:0005515 IPI
    oxidoreductase activity GO:0016491 IDA
    palmitoyl-CoA 9-desaturase activity GO:0032896 IBA
Biological Process
    unsaturated fatty acid biosynthetic process GO:0006636 IBA
    unsaturated fatty acid biosynthetic process GO:0006636 IDA
    response to fatty acid GO:0070542 IBA
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    monounsaturated fatty acid biosynthetic process GO:1903966 IBA
Subcellular Localization
    nucleolus GO:0005730 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified SCD in sEVs
1
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SCD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 PANK1  
Affinity Capture-MS Homo sapiens
3 SSR4 6748
Co-fractionation Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 SNX27 81609
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 FASN 2194
Positive Genetic Homo sapiens
8 SSR1 6745
Proximity Label-MS Homo sapiens
9 TTYH1  
Affinity Capture-MS Homo sapiens
10 PANK2 80025
Affinity Capture-MS Homo sapiens
11 SLC25A3 5250
Co-fractionation Homo sapiens
12 FBXL6  
Affinity Capture-MS Homo sapiens
13 VAPA 9218
Affinity Capture-MS Homo sapiens
14 PGRMC1 10857
Affinity Capture-MS Homo sapiens
15 TTC27 55622
Affinity Capture-MS Homo sapiens
16 ESF1 51575
Co-fractionation Homo sapiens
17 UQCRC2 7385
Co-fractionation Homo sapiens
18 BCAP31 10134
Affinity Capture-MS Homo sapiens
19 RPL14 9045
Co-fractionation Homo sapiens
20 CUL3 8452
Affinity Capture-MS Homo sapiens
21 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
22 HSD17B11 51170
Proximity Label-MS Homo sapiens
23 UBC 7316
Reconstituted Complex Homo sapiens
24 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
25 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
26 RB1CC1 9821
Affinity Capture-MS Homo sapiens
27 PARK2  
Affinity Capture-MS Homo sapiens
28 DNAJC25 548645
Proximity Label-MS Homo sapiens
29 AMFR 267
Affinity Capture-MS Homo sapiens
30 HSD17B12 51144
Co-fractionation Homo sapiens
31 DDX58 23586
Affinity Capture-RNA Homo sapiens
32 RTN4 57142
Affinity Capture-MS Homo sapiens
33 TTC30B  
Affinity Capture-MS Homo sapiens
34 RPS3 6188
Co-fractionation Homo sapiens
35 MAPK6  
Affinity Capture-MS Homo sapiens
36 C12orf49  
Negative Genetic Homo sapiens
37 ARHGAP15 55843
Affinity Capture-MS Homo sapiens
38 PPP2R1A 5518
Co-fractionation Homo sapiens
39 SEC62 7095
Proximity Label-MS Homo sapiens
40 VCP 7415
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
41 VAPB 9217
Co-fractionation Homo sapiens
42 RAB7A 7879
Proximity Label-MS Homo sapiens
43 GPR182  
Affinity Capture-MS Homo sapiens
44 STT3B 201595
Affinity Capture-MS Homo sapiens
45 RPN1 6184
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 KRAS 3845
Negative Genetic Homo sapiens
47 UBE3B 89910
Affinity Capture-MS Homo sapiens
48 C9orf72  
Affinity Capture-MS Homo sapiens
49 CGRRF1  
Affinity Capture-MS Homo sapiens
50 CKAP4 10970
Proximity Label-MS Homo sapiens
51 PANK3 79646
Affinity Capture-MS Homo sapiens
52 DDOST 1650
Affinity Capture-MS Homo sapiens
53 RPN2 6185
Affinity Capture-MS Homo sapiens
54 ESYT1 23344
Affinity Capture-MS Homo sapiens
55 APP 351
Reconstituted Complex Homo sapiens
56 NEDD4L 23327
Reconstituted Complex Homo sapiens
57 NSUN5  
Co-fractionation Homo sapiens
58 HUWE1 10075
Affinity Capture-MS Homo sapiens
59 OST4  
Affinity Capture-MS Homo sapiens
60 CCDC47 57003
Affinity Capture-MS Homo sapiens
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