ExoCarta: Gene summary

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Gene description for SGSH
Gene name	N-sulfoglucosamine sulfohydrolase
Gene symbol	SGSH
Other names/aliases	HSS MPS3A SFMD
Species	Homo sapiens
Database cross references - SGSH
ExoCarta	ExoCarta_6448
Vesiclepedia	VP_6448
Entrez Gene	6448
HGNC	10818
MIM	605270
UniProt	P51688
SGSH identified in sEVs derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Colorectal cancer cells	34887515
Endothelial cells	26027894

Gene ontology annotations for SGSH

Molecular Function
	protein binding	GO:0005515	IPI
	sulfuric ester hydrolase activity	GO:0008484	IEA
	N-sulfoglucosamine sulfohydrolase activity	GO:0016250	IBA
	N-sulfoglucosamine sulfohydrolase activity	GO:0016250	IDA
	N-sulfoglucosamine sulfohydrolase activity	GO:0016250	TAS
	metal ion binding	GO:0046872	IEA
Biological Process
	glycosaminoglycan catabolic process	GO:0006027	IBA
	glycosaminoglycan catabolic process	GO:0006027	IDA
	determination of adult lifespan	GO:0008340	IEA
	heparan sulfate proteoglycan catabolic process	GO:0030200	IBA
	heparan sulfate proteoglycan catabolic process	GO:0030200	IMP
	heparan sulfate proteoglycan catabolic process	GO:0030200	TAS
	motor behavior	GO:0061744	IEA
Subcellular Localization
	lysosome	GO:0005764	IDA
	lysosomal lumen	GO:0043202	TAS
	extracellular exosome	GO:0070062	HDA

Experiment description of studies that identified SGSH in sEVs

Experiment ID

494

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD151\|CD63\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Osteoarthritic cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

496

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Healthy cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Experiment ID

226

MISEV standards
✔ EM	Biophysical techniques
✔ GAPDH\|CD9\|FLOT1	Enriched markers
✔ LMNA\|H2AFX\|ATP5A1\|TOMM20	Negative markers
✘	Particle analysis

Identified molecule

mRNA

Identification method

Small RNA sequencing (Illumina HiSeq 2000 (Solexa)

PubMed ID

26027894

Organism

Homo sapiens

Experiment description

"Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"

Authors

"Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"

Journal name

Journal of Extracellular Vesicles

Publication year

2015

Sample

Endothelial cells

Sample name

HMEC-1

Isolation/purification methods

Differential ultracentrifugation
Sucrose density gradient

Flotation density

1.10 g/mL

Molecules identified in the study

miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA

Methods used in the study

Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting

Protein-protein interactions for SGSH

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

LY86

Affinity Capture-MS

Homo sapiens

NGLY1

55768

Affinity Capture-MS

Homo sapiens

RRM1

6240

Co-fractionation

Homo sapiens

PPT1

5538

Co-fractionation

Homo sapiens

SKP1

6500

Affinity Capture-MS

Homo sapiens

OXCT1

5019

Co-fractionation

Homo sapiens

NAAA

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SIAE

54414

Affinity Capture-MS

Homo sapiens

CANX

821

Affinity Capture-MS

Homo sapiens

ARSA

410

Affinity Capture-MS

Homo sapiens

S100A16

140576

Co-fractionation

Homo sapiens

GGH

8836

Affinity Capture-MS

Homo sapiens

FBXO21

Affinity Capture-MS

Homo sapiens

PLAUR

5329

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRG2

Affinity Capture-MS

Homo sapiens

MFAP4

4239

Affinity Capture-MS

Homo sapiens

PI15

Affinity Capture-MS

Homo sapiens

IGF2R

3482

Affinity Capture-MS

Homo sapiens

FBXO6

26270

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

IL5RA

Affinity Capture-MS

Homo sapiens

HSPA5

3309

Affinity Capture-MS

Homo sapiens

TMEM25

Affinity Capture-MS

Homo sapiens

IDS

Affinity Capture-MS

Homo sapiens

SDF2L1

23753

Affinity Capture-MS

Homo sapiens

FBXO2

26232

Affinity Capture-MS

Homo sapiens

OGT

8473

Reconstituted Complex

Homo sapiens

CRISP2

Affinity Capture-MS

Homo sapiens

DNAJB9

4189

Affinity Capture-MS

Homo sapiens

C1QBP

708

Co-fractionation

Homo sapiens

SMAD2

4087

Affinity Capture-MS

Homo sapiens

NMD3

51068

Affinity Capture-MS

Homo sapiens

SUMF1

285362

Affinity Capture-MS

Homo sapiens

PRDX2

7001

Co-fractionation

Homo sapiens

INSL4

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which SGSH is involved

Pathway	Evidence	Source
Disease	TAS	Reactome
Diseases of carbohydrate metabolism	TAS	Reactome
Diseases of metabolism	TAS	Reactome
Glycosaminoglycan metabolism	TAS	Reactome
Heparan sulfate/heparin (HS-GAG) metabolism	TAS	Reactome
HS-GAG degradation	TAS	Reactome
Metabolism	TAS	Reactome
Metabolism of carbohydrates	TAS	Reactome
MPS IIIA - Sanfilippo syndrome A	TAS	Reactome
Mucopolysaccharidoses	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here