Gene description for SLC5A3
Gene name solute carrier family 5 (sodium/myo-inositol cotransporter), member 3
Gene symbol SLC5A3
Other names/aliases BCW2
SMIT
SMIT1
SMIT2
Species Homo sapiens
 Database cross references - SLC5A3
ExoCarta ExoCarta_6526
Vesiclepedia VP_6526
Entrez Gene 6526
HGNC 11038
MIM 600444
 SLC5A3 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC5A3
Molecular Function
    myo-inositol transmembrane transporter activity GO:0005365 ISS
    myo-inositol:sodium symporter activity GO:0005367 IDA
    myo-inositol:sodium symporter activity GO:0005367 ISS
    myo-inositol:sodium symporter activity GO:0005367 TAS
    D-glucose:sodium symporter activity GO:0005412 IBA
    D-glucose:sodium symporter activity GO:0005412 ISS
    protein binding GO:0005515 IPI
    pentose transmembrane transporter activity GO:0015146 ISS
    fucose transmembrane transporter activity GO:0015150 ISS
    polyol transmembrane transporter activity GO:0015166 ISS
    potassium channel regulator activity GO:0015459 IDA
    transmembrane transporter binding GO:0044325 IPI
Biological Process
    inositol metabolic process GO:0006020 IBA
    pentose transmembrane transport GO:0015750 ISS
    fucose transmembrane transport GO:0015756 ISS
    polyol transmembrane transport GO:0015791 ISS
    myo-inositol transport GO:0015798 IBA
    myo-inositol transport GO:0015798 ISS
    myo-inositol transport GO:0015798 TAS
    sodium ion transmembrane transport GO:0035725 IEA
    positive regulation of catalytic activity GO:0043085 ISS
    transport across blood-brain barrier GO:0150104 NAS
    positive regulation of reactive oxygen species biosynthetic process GO:1903428 ISS
    D-glucose transmembrane transport GO:1904659 ISS
    myo-inositol import across plasma membrane GO:1904679 ISS
    positive regulation of protein localization to membrane GO:1905477 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    basolateral plasma membrane GO:0016323 IEA
    apical plasma membrane GO:0016324 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified SLC5A3 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC5A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC37A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 RAB35 11021
Proximity Label-MS Homo sapiens
4 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ADORA2B  
Affinity Capture-MS Homo sapiens
6 EDNRA 1909
Affinity Capture-MS Homo sapiens
7 DIO3  
Affinity Capture-MS Homo sapiens
8 PTH1R  
Affinity Capture-MS Homo sapiens
9 TTYH1  
Affinity Capture-MS Homo sapiens
10 GPR17 2840
Affinity Capture-MS Homo sapiens
11 LAMP3  
Proximity Label-MS Homo sapiens
12 SLC2A4  
Affinity Capture-MS Homo sapiens
13 MFSD4  
Affinity Capture-MS Homo sapiens
14 LPAR6  
Affinity Capture-MS Homo sapiens
15 LAMP1 3916
Proximity Label-MS Homo sapiens
16 TMEM184A  
Affinity Capture-MS Homo sapiens
17 RARRES3  
Affinity Capture-MS Homo sapiens
18 CRELD1 78987
Affinity Capture-MS Homo sapiens
19 HS2ST1 9653
Affinity Capture-MS Homo sapiens
20 GPR12  
Affinity Capture-MS Homo sapiens
21 GCGR  
Affinity Capture-MS Homo sapiens
22 LAMP2 3920
Proximity Label-MS Homo sapiens
23 SLC5A6 8884
Affinity Capture-MS Homo sapiens
24 ARF6 382
Proximity Label-MS Homo sapiens
25 AQP3  
Affinity Capture-MS Homo sapiens
26 C5AR1  
Affinity Capture-MS Homo sapiens
27 OR4N2  
Affinity Capture-MS Homo sapiens
28 LPAR1 1902
Affinity Capture-MS Homo sapiens
29 FN1 2335
Affinity Capture-MS Homo sapiens
30 VSIG4  
Affinity Capture-MS Homo sapiens
31 DNAJC5 80331
Proximity Label-MS Homo sapiens
32 TSPAN8 7103
Affinity Capture-MS Homo sapiens
33 B3GAT1  
Proximity Label-MS Homo sapiens
34 RAB2A 5862
Proximity Label-MS Homo sapiens
35 FPR2  
Affinity Capture-MS Homo sapiens
36 GPR45  
Affinity Capture-MS Homo sapiens
37 CMTM5  
Affinity Capture-MS Homo sapiens
38 GPR182  
Affinity Capture-MS Homo sapiens
39 IQCB1  
Affinity Capture-MS Homo sapiens
40 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 GPR88  
Affinity Capture-MS Homo sapiens
42 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KRAS 3845
Proximity Label-MS Homo sapiens
44 P2RY12 64805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CAV1 857
Proximity Label-MS Homo sapiens
46 GPR173  
Affinity Capture-MS Homo sapiens
47 RHOB 388
Proximity Label-MS Homo sapiens
48 ZACN  
Affinity Capture-MS Homo sapiens
49 CACNG6  
Affinity Capture-MS Homo sapiens
50 AVPR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here