Experiment description of studies that identified POTEJ in sEVs

1 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 193 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ PHB Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 194 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of basement membrane nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 195 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of early IgA nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for POTEJ

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ANLN 54443 Affinity Capture-MS Homo sapiens 2 RELA 5970 Affinity Capture-MS Homo sapiens 3 TFCP2 7024 Affinity Capture-MS Homo sapiens 4 LGR4 55366 Affinity Capture-MS Homo sapiens 5 KIF14 9928 Affinity Capture-MS Homo sapiens 6 NR3C1 2908 Proximity Label-MS Homo sapiens 7 RIN3   Affinity Capture-MS Homo sapiens 8 ECT2 1894 Affinity Capture-MS Homo sapiens 9 RIT1 6016 Negative Genetic Homo sapiens 10 ACTA2 59 Cross-Linking-MS (XL-MS) Homo sapiens 11 ACTC1 70 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 12 ACTBL2 345651 Cross-Linking-MS (XL-MS) Homo sapiens 13 KIF20A 10112 Affinity Capture-MS Homo sapiens 14 CAP2 10486 Affinity Capture-MS Homo sapiens 15 EIF4A3 9775 Affinity Capture-MS Homo sapiens 16 VARS2   Co-fractionation Homo sapiens 17 AURKB 9212 Affinity Capture-MS Homo sapiens 18 POTEE 445582 Cross-Linking-MS (XL-MS) Homo sapiens 19 CUL9   Affinity Capture-MS Homo sapiens 20 ACTA1 58 Cross-Linking-MS (XL-MS) Homo sapiens 21 MYC   Affinity Capture-MS Homo sapiens 22 YAP1 10413 Affinity Capture-MS Homo sapiens 23 PFN3   Affinity Capture-MS Homo sapiens 24 DDX24 57062 Co-fractionation Homo sapiens 25 PRC1 9055 Affinity Capture-MS Homo sapiens 26 ACTG2 72 Cross-Linking-MS (XL-MS) Homo sapiens 27 ACTB 60 Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 28 MAGOH 4116 Affinity Capture-MS Homo sapiens 29 POTEI 653269 Cross-Linking-MS (XL-MS) Homo sapiens 30 BCL7A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 ACTG1 71 Cross-Linking-MS (XL-MS) Homo sapiens View the network

Pathways in which POTEJ is involved

No pathways found