Experiment description of studies that identified POTEI in sEVs

1 Experiment ID 20 MISEV standards ✔ EM Biophysical techniques ✔ HSP90|CD63|CD81|LAMP1 Enriched markers ✔ GOLGA2|cytochrome c Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17956143     Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells. Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" Journal name JPR Publication year 2007 Sample Colorectal cancer cells Sample name HT29 Isolation/purification methods Differential centrifugation Sucrose density gradient Diafiltration Flotation density 1.16 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

2 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

3 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

4 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 193 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ PHB Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 194 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of basement membrane nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 195 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of early IgA nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for POTEI

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SCIN 85477 Affinity Capture-MS Homo sapiens 2 RRN3   Affinity Capture-MS Homo sapiens 3 C20orf203   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 ABRA   Affinity Capture-MS Homo sapiens 5 SYNGR1 9145 Affinity Capture-MS Homo sapiens 6 MME 4311 Affinity Capture-MS Homo sapiens 7 SPERT   Affinity Capture-MS Homo sapiens 8 GC 2638 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 CAP1 10487 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 ACTC1 70 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 11 DTNA   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 HSD17B3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 CAP2 10486 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 DKK1 22943 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 FGFR3 2261 Affinity Capture-MS Homo sapiens 16 POLH   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 ARL6IP6   Affinity Capture-MS Homo sapiens 18 GGT5 2687 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 S100A4 6275 Affinity Capture-MS Homo sapiens 20 DHX33   Affinity Capture-MS Homo sapiens 21 WASH3P   Affinity Capture-MS Homo sapiens 22 ACTG2 72 Cross-Linking-MS (XL-MS) Homo sapiens 23 IL1RL2 8808 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 LRRC20   Affinity Capture-MS Homo sapiens 25 MALSU1   Affinity Capture-MS Homo sapiens 26 TEX19   Affinity Capture-MS Homo sapiens 27 AASDHPPT 60496 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 TFCP2 7024 Affinity Capture-MS Homo sapiens 29 MYCN   Affinity Capture-MS Homo sapiens 30 HPCAL4   Affinity Capture-MS Homo sapiens 31 EIF1B   Affinity Capture-MS Homo sapiens 32 ACTA2 59 Cross-Linking-MS (XL-MS) Homo sapiens 33 FAM173B   Affinity Capture-MS Homo sapiens 34 NUS1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 HMGN2 3151 Cross-Linking-MS (XL-MS) Homo sapiens 36 EMP3 2014 Affinity Capture-MS Homo sapiens 37 TMED5 50999 Affinity Capture-MS Homo sapiens 38 TMEM134   Affinity Capture-MS Homo sapiens 39 ACTB 60 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 40 CCDC22 28952 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 FIGNL1 63979 Affinity Capture-MS Homo sapiens 42 PHACTR2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 YAP1 10413 Affinity Capture-MS Homo sapiens 44 DUSP23 54935 Affinity Capture-MS Homo sapiens 45 ACTG1 71 Cross-Linking-MS (XL-MS) Homo sapiens 46 FGFR1OP   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 47 MTSS1L 92154 Affinity Capture-MS Homo sapiens 48 AFM 173 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 NID2 22795 Affinity Capture-MS Homo sapiens 50 RBBP6 5930 Affinity Capture-MS Homo sapiens 51 BRF1   Affinity Capture-MS Homo sapiens 52 HADHB 3032 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 NDUFA4L2 56901 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 54 RLIM 51132 Affinity Capture-MS Homo sapiens 55 RPRD2   Affinity Capture-MS Homo sapiens 56 ACTBL2 345651 Cross-Linking-MS (XL-MS) Homo sapiens 57 OSER1   Affinity Capture-MS Homo sapiens 58 IKZF1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 59 CFI 3426 Affinity Capture-MS Homo sapiens 60 CYP51A1 1595 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 61 MYO6 4646 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 62 BAG1 573 Affinity Capture-MS Homo sapiens 63 C1QTNF1 114897 Affinity Capture-MS Homo sapiens 64 TMOD3 29766 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 65 CDKL4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 66 MARCH9   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 67 DDRGK1 65992 Affinity Capture-MS Homo sapiens 68 ADAT3 113179 Affinity Capture-MS Homo sapiens 69 PLEKHG3 26030 Affinity Capture-MS Homo sapiens 70 GSN 2934 Affinity Capture-MS Homo sapiens 71 PLEKHF1 79156 Affinity Capture-MS Homo sapiens 72 RNASE3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 73 SLC25A11 8402 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 74 PDCL3 79031 Affinity Capture-MS Homo sapiens 75 PARVG 64098 Affinity Capture-MS Homo sapiens 76 PIGO   Affinity Capture-MS Homo sapiens 77 SSH1   Affinity Capture-MS Homo sapiens 78 POTEJ 653781 Cross-Linking-MS (XL-MS) Homo sapiens 79 TPMT 7172 Affinity Capture-MS Homo sapiens 80 SMNDC1   Affinity Capture-MS Homo sapiens 81 POTEE 445582 Cross-Linking-MS (XL-MS) Homo sapiens 82 CYTH1 9267 Affinity Capture-MS Homo sapiens 83 EPB41L2 2037 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 84 PCGF3   Affinity Capture-MS Homo sapiens 85 ACTA1 58 Cross-Linking-MS (XL-MS) Homo sapiens 86 TEX264 51368 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 87 KRT38 8687 Affinity Capture-MS Homo sapiens 88 SPPL2B 56928 Affinity Capture-MS Homo sapiens 89 LPAR2 9170 Affinity Capture-MS Homo sapiens 90 TPST2 8459 Affinity Capture-MS Homo sapiens 91 CCDC40   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 92 MAEA 10296 Affinity Capture-MS Homo sapiens View the network

Pathways in which POTEI is involved

No pathways found