Gene description for SCIN
Gene name scinderin
Gene symbol SCIN
Other names/aliases -
Species Homo sapiens
 Database cross references - SCIN
ExoCarta ExoCarta_85477
Vesiclepedia VP_85477
Entrez Gene 85477
HGNC 21695
MIM 613416
UniProt Q9Y6U3  
 SCIN identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for SCIN
Molecular Function
    phosphatidylserine binding GO:0001786 ISS
    actin binding GO:0003779 ISS
    actin binding GO:0003779 TAS
    calcium ion binding GO:0005509 ISS
    calcium ion binding GO:0005509 TAS
    1-phosphatidylinositol binding GO:0005545 TAS
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IBA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISS
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 ISS
Biological Process
    central nervous system development GO:0007417 IBA
    actin polymerization or depolymerization GO:0008154 IBA
    negative regulation of cell population proliferation GO:0008285 IMP
    calcium-ion regulated exocytosis GO:0017156 ISS
    cell projection assembly GO:0030031 IBA
    regulation of chondrocyte differentiation GO:0032330 ISS
    sequestering of actin monomers GO:0042989 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    actin nucleation GO:0045010 ISS
    positive regulation of megakaryocyte differentiation GO:0045654 IMP
    actin filament severing GO:0051014 IBA
    actin filament severing GO:0051014 IMP
    barbed-end actin filament capping GO:0051016 IBA
    positive regulation of secretion GO:0051047 ISS
    positive regulation of actin nucleation GO:0051127 IMP
    actin filament capping GO:0051693 IMP
Subcellular Localization
    podosome GO:0002102 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    brush border GO:0005903 IEA
    cell cortex GO:0005938 ISS
    actin cytoskeleton GO:0015629 IBA
    cell projection GO:0042995 IEA
    extracellular exosome GO:0070062 HDA
    anchoring junction GO:0070161 IEA
 Experiment description of studies that identified SCIN in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SCIN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
2 LSP1 4046
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 ACTA2 59
Affinity Capture-MS Homo sapiens
5 TMOD2 29767
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MSH2 4436
Co-fractionation Homo sapiens
7 XPO1 7514
Co-fractionation Homo sapiens
8 POTEI 653269
Affinity Capture-MS Homo sapiens
9 PFN1 5216
Proximity Label-MS Homo sapiens
10 ATG9A 79065
Proximity Label-MS Homo sapiens
11 BTD 686
Co-fractionation Homo sapiens
12 ACTC1 70
Affinity Capture-MS Homo sapiens
13 UBA1 7317
Co-fractionation Homo sapiens
14 ACTBL2 345651
Affinity Capture-MS Homo sapiens
15 KIF20A 10112
Affinity Capture-MS Homo sapiens
16 POTEF 728378
Affinity Capture-MS Homo sapiens
17 ACTR1B 10120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GPAM 57678
Affinity Capture-MS Homo sapiens
19 INPP4B 8821
Co-fractionation Homo sapiens
20 TMOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 G6PD 2539
Co-fractionation Homo sapiens
22 GARS 2617
Co-fractionation Homo sapiens
23 ACTA1 58
Reconstituted Complex Homo sapiens
24 SSH1  
Affinity Capture-MS Homo sapiens
25 FBXO30 84085
Affinity Capture-MS Homo sapiens
26 BCL7C  
Affinity Capture-MS Homo sapiens
27 RAB7A 7879
Proximity Label-MS Homo sapiens
28 TMOD3 29766
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 GRXCR1  
Affinity Capture-MS Homo sapiens
30 ACTB 60
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 OBSL1 23363
Affinity Capture-MS Homo sapiens
32 BCL7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ACTG1 71
Affinity Capture-MS Homo sapiens
34 CCDC8  
Affinity Capture-MS Homo sapiens
35 SLC30A5 64924
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SCIN is involved
No pathways found





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