Gene description for SMPD1
Gene name sphingomyelin phosphodiesterase 1, acid lysosomal
Gene symbol SMPD1
Other names/aliases ASM
ASMASE
NPD
Species Homo sapiens
 Database cross references - SMPD1
ExoCarta ExoCarta_6609
Vesiclepedia VP_6609
Entrez Gene 6609
HGNC 11120
MIM 607608
UniProt P17405  
 SMPD1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMPD1
Molecular Function
    sphingomyelin phosphodiesterase activity GO:0004767 IDA
    sphingomyelin phosphodiesterase activity GO:0004767 TAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    hydrolase activity, acting on glycosyl bonds GO:0016798 IEA
    phosphatidylcholine phospholipase C activity GO:0034480 IEA
    acid sphingomyelin phosphodiesterase activity GO:0061750 IBA
    acid sphingomyelin phosphodiesterase activity GO:0061750 IDA
Biological Process
    plasma membrane repair GO:0001778 IDA
    sphingomyelin metabolic process GO:0006684 TAS
    sphingomyelin catabolic process GO:0006685 IBA
    sphingomyelin catabolic process GO:0006685 IDA
    signal transduction GO:0007165 TAS
    nervous system development GO:0007399 TAS
    cholesterol metabolic process GO:0008203 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to virus GO:0009615 IDA
    response to ionizing radiation GO:0010212 IMP
    termination of signal transduction GO:0023021 IMP
    response to type I interferon GO:0034340 IDA
    response to tumor necrosis factor GO:0034612 IDA
    cellular response to UV GO:0034644 IDA
    wound healing GO:0042060 IDA
    response to cocaine GO:0042220 IEA
    positive regulation of apoptotic process GO:0043065 IMP
    negative regulation of MAPK cascade GO:0043409 IMP
    positive regulation of endocytosis GO:0045807 IDA
    glycosphingolipid catabolic process GO:0046479 TAS
    ceramide biosynthetic process GO:0046513 IBA
    ceramide biosynthetic process GO:0046513 IDA
    ceramide biosynthetic process GO:0046513 IMP
    positive regulation of viral entry into host cell GO:0046598 IDA
    symbiont entry into host cell GO:0046718 IDA
    symbiont entry into host cell GO:0046718 IDA
    symbiont entry into host cell GO:0046718 IDA
    response to interleukin-1 GO:0070555 IDA
    cellular response to calcium ion GO:0071277 IDA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosome GO:0005764 IDA
    endosome GO:0005768 IDA
    lipid droplet GO:0005811 IEA
    plasma membrane GO:0005886 IDA
    endolysosome GO:0036019 IDA
    lamellar body GO:0042599 IEA
    lysosomal lumen GO:0043202 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SMPD1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMPD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C7orf34  
Affinity Capture-MS Homo sapiens
2 FASN 2194
Negative Genetic Homo sapiens
3 ALDH3A2 224
Affinity Capture-MS Homo sapiens
4 CLGN 1047
Affinity Capture-MS Homo sapiens
5 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 VTI1A 143187
Affinity Capture-MS Homo sapiens
7 MGRN1 23295
Affinity Capture-MS Homo sapiens
8 ANXA7 310
Two-hybrid Homo sapiens
9 PDGFRA 5156
Affinity Capture-MS Homo sapiens
10 ERLEC1 27248
Affinity Capture-MS Homo sapiens
11 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
12 TMEM126A 84233
Affinity Capture-MS Homo sapiens
13 OS9 10956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 INSL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 GDAP1 54332
Affinity Capture-MS Homo sapiens
16 APEX1 328
Affinity Capture-RNA Homo sapiens
17 MOV10 4343
Affinity Capture-RNA Homo sapiens
18 CNTNAP3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 FBXO27  
Affinity Capture-MS Homo sapiens
20 SCGB2A2  
Affinity Capture-MS Homo sapiens
21 ANXA4 307
Two-hybrid Homo sapiens
22 IGF2R 3482
Affinity Capture-MS Homo sapiens
23 SEL1L 6400
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SLC27A2 11001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ALG11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NR1H2  
Two-hybrid Homo sapiens
27 DUSP23 54935
Two-hybrid Homo sapiens
28 ATRN 8455
Affinity Capture-MS Homo sapiens
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