Gene description for Dnm1l
Gene name dynamin 1-like
Gene symbol Dnm1l
Other names/aliases 6330417M19Rik
AI450666
Dlp1
Dnmlp1
Drp1
python
Species Mus musculus
 Database cross references - Dnm1l
ExoCarta ExoCarta_74006
Vesiclepedia VP_74006
Entrez Gene 74006
UniProt Q8K1M6  
 Dnm1l identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnm1l
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    microtubule binding GO:0008017 IBA
    lipid binding GO:0008289 IEA
    clathrin binding GO:0030276 IEA
    clathrin binding GO:0030276 ISO
    GTP-dependent protein binding GO:0030742 IEA
    GTP-dependent protein binding GO:0030742 ISO
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IPI
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    BH2 domain binding GO:0051433 IEA
    BH2 domain binding GO:0051433 ISO
    protein serine/threonine kinase binding GO:0120283 IEA
    protein serine/threonine kinase binding GO:0120283 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    mitochondrial fission GO:0000266 IBA
    mitochondrial fission GO:0000266 IMP
    mitochondrial fission GO:0000266 IMP
    mitochondrial fission GO:0000266 ISO
    mitochondrial fission GO:0000266 ISO
    mitochondrial fission GO:0000266 ISS
    release of cytochrome c from mitochondria GO:0001836 ISO
    calcium ion transport GO:0006816 IMP
    endocytosis GO:0006897 IBA
    mitochondrion organization GO:0007005 IMP
    mitochondrion organization GO:0007005 ISO
    mitochondrion organization GO:0007005 ISO
    regulation of gene expression GO:0010468 IMP
    negative regulation of mitochondrial fusion GO:0010637 IEA
    negative regulation of mitochondrial fusion GO:0010637 ISO
    regulation of mitochondrion organization GO:0010821 IMP
    regulation of mitochondrion organization GO:0010821 ISO
    peroxisome fission GO:0016559 IBA
    peroxisome fission GO:0016559 IMP
    peroxisome fission GO:0016559 ISO
    peroxisome fission GO:0016559 ISS
    response to endoplasmic reticulum stress GO:0034976 IEA
    response to endoplasmic reticulum stress GO:0034976 ISO
    synaptic vesicle recycling via endosome GO:0036466 IEA
    synaptic vesicle recycling via endosome GO:0036466 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IBA
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IEA
    mitochondrial fragmentation involved in apoptotic process GO:0043653 ISO
    intracellular distribution of mitochondria GO:0048312 IBA
    intracellular distribution of mitochondria GO:0048312 IEA
    intracellular distribution of mitochondria GO:0048312 ISO
    synaptic vesicle endocytosis GO:0048488 IEA
    synaptic vesicle endocytosis GO:0048488 ISO
    rhythmic process GO:0048511 IEA
    positive regulation of protein secretion GO:0050714 IEA
    positive regulation of protein secretion GO:0050714 ISO
    protein complex oligomerization GO:0051259 ISO
    protein complex oligomerization GO:0051259 ISS
    heart contraction GO:0060047 IMP
    positive regulation of dendritic spine morphogenesis GO:0061003 IEA
    positive regulation of dendritic spine morphogenesis GO:0061003 ISO
    membrane fusion GO:0061025 ISO
    protein-containing complex assembly GO:0065003 ISO
    necroptotic process GO:0070266 ISO
    protein localization to mitochondrion GO:0070585 IMP
    protein localization to mitochondrion GO:0070585 ISO
    cellular response to lipid GO:0071396 IEA
    cellular response to hypoxia GO:0071456 IEA
    positive regulation of neutrophil chemotaxis GO:0090023 IEA
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
    positive regulation of mitochondrial fission GO:0090141 IEA
    positive regulation of mitochondrial fission GO:0090141 ISO
    mitochondrial membrane fission GO:0090149 IEA
    mitochondrial membrane fission GO:0090149 ISO
    positive regulation of release of cytochrome c from mitochondria GO:0090200 ISO
    mitocytosis GO:0160040 IMP
    regulation of peroxisome organization GO:1900063 IMP
    regulation of peroxisome organization GO:1900063 ISO
    positive regulation of synaptic vesicle endocytosis GO:1900244 IEA
    positive regulation of synaptic vesicle endocytosis GO:1900244 ISO
    regulation of mitophagy GO:1901524 IEA
    regulation of mitophagy GO:1901524 ISO
    regulation of ATP metabolic process GO:1903578 IMP
    regulation of ubiquitin protein ligase activity GO:1904666 IMP
    response to flavonoid GO:1905395 IEA
    response to hypobaric hypoxia GO:1990910 IEA
    positive regulation of synaptic vesicle exocytosis GO:2000302 IEA
    positive regulation of synaptic vesicle exocytosis GO:2000302 ISO
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IMP
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 ISO
    lysosome GO:0005764 IEA
    late endosome GO:0005770 IEA
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 ISO
    peroxisome GO:0005777 ISS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IMP
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISO
    brush border GO:0005903 IDA
    clathrin-coated pit GO:0005905 IEA
    clathrin-coated pit GO:0005905 ISO
    postsynaptic density GO:0014069 IEA
    membrane GO:0016020 IBA
    synaptic vesicle membrane GO:0030672 IEA
    synaptic vesicle membrane GO:0030672 ISO
    mitochondrial membrane GO:0031966 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    presynaptic endocytic zone membrane GO:0098835 IEA
    presynaptic endocytic zone membrane GO:0098835 ISO
    mitochondrion-derived vesicle GO:0099073 IEA
    mitochondrion-derived vesicle GO:0099073 ISO
    secretory vesicle GO:0099503 ISO
 Experiment description of studies that identified Dnm1l in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnm1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kcna3  
Affinity Capture-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Syngap1  
Affinity Capture-MS Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Foxp3  
Affinity Capture-MS Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Akap1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
8 Sirt3  
Co-fractionation Mus musculus
9 Mapt  
Affinity Capture-MS Mus musculus
10 CSNK2A1 1457
Two-hybrid Homo sapiens
11 Unk  
Affinity Capture-RNA Mus musculus
12 Dnm1l 74006
Two-hybrid Mus musculus
Two-hybrid Mus musculus
13 Lrrk2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
14 Cyfip1 20430
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Dnm1l is involved
PathwayEvidenceSource
Apoptosis IEA Reactome
Apoptotic execution phase IEA Reactome
Programmed Cell Death IEA Reactome





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