Gene description for Arpc2
Gene name actin related protein 2/3 complex, subunit 2
Gene symbol Arpc2
Other names/aliases 2210023N03Rik
34kDa
p34-Arc
Species Mus musculus
 Database cross references - Arpc2
ExoCarta ExoCarta_76709
Vesiclepedia VP_76709
Entrez Gene 76709
UniProt Q9CVB6  
 Arpc2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arpc2
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    protein binding GO:0005515 IPI
    glutamate receptor binding GO:0035254 ISO
    protein-containing complex binding GO:0044877 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IEA
    actin filament binding GO:0051015 ISO
    ATPase binding GO:0051117 ISO
Biological Process
    positive regulation of lamellipodium assembly GO:0010592 IEA
    positive regulation of lamellipodium assembly GO:0010592 ISO
    positive regulation of smooth muscle cell migration GO:0014911 ISO
    actin filament polymerization GO:0030041 IEA
    positive regulation of actin filament polymerization GO:0030838 IDA
    regulation of actin cytoskeleton organization GO:0032956 ISO
    Arp2/3 complex-mediated actin nucleation GO:0034314 IBA
    Arp2/3 complex-mediated actin nucleation GO:0034314 IEA
    Arp2/3 complex-mediated actin nucleation GO:0034314 ISO
    cellular response to platelet-derived growth factor stimulus GO:0036120 ISO
    positive regulation of filopodium assembly GO:0051491 ISO
    protein kinase C signaling GO:0070528 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    positive regulation of podosome assembly GO:0071803 ISO
    cellular response to rapamycin GO:0072752 ISO
    positive regulation of protein targeting to membrane GO:0090314 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    positive regulation of barbed-end actin filament capping GO:2000814 ISO
Subcellular Localization
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    endosome GO:0005768 IDA
    Arp2/3 protein complex GO:0005885 IBA
    Arp2/3 protein complex GO:0005885 ISO
    Arp2/3 protein complex GO:0005885 ISS
    Arp2/3 protein complex GO:0005885 TAS
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 IDA
    cell surface GO:0009986 ISO
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    secretory granule GO:0030141 ISO
    growth cone GO:0030426 ISO
    synaptic vesicle membrane GO:0030672 IDA
    cell leading edge GO:0031252 IDA
    cell leading edge GO:0031252 ISO
    filamentous actin GO:0031941 ISO
    ruffle membrane GO:0032587 ISO
    site of double-strand break GO:0035861 ISS
    muscle cell projection membrane GO:0036195 IDA
    neuron projection GO:0043005 IEA
    dendritic spine GO:0043197 ISO
    dendritic shaft GO:0043198 ISO
    perikaryon GO:0043204 ISO
    axon terminus GO:0043679 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    podosome core GO:0061825 ISO
    podosome ring GO:0061826 ISO
    concave side of sperm head GO:0061830 ISO
    actin filament branch point GO:0061834 ISO
    ventral surface of cell GO:0061835 ISO
    peripheral region of growth cone GO:0090725 ISO
    apical dendrite GO:0097440 ISO
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    presynapse GO:0098793 IDA
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Arpc2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arpc2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Mycbp2 105689
Affinity Capture-MS Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Mtr  
Co-fractionation Mus musculus
8 Nsun2 28114
Co-fractionation Mus musculus
9 Tpd52l2  
Co-fractionation Mus musculus
10 Thg1l  
Co-fractionation Mus musculus
11 Anp32b 67628
Co-fractionation Mus musculus
12 Unk  
Affinity Capture-RNA Mus musculus
13 VASP 7408
Affinity Capture-MS Homo sapiens
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