Experiment description of studies that identified ZFAND5 in sEVs

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

Protein-protein interactions for ZFAND5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ATP6V1H 51606 Co-fractionation Homo sapiens 2 SQSTM1 8878 Proximity Label-MS Homo sapiens Co-localization Homo sapiens 3 PSMA1 5682 Affinity Capture-Western Homo sapiens 4 UBC 7316 Co-crystal Structure Homo sapiens Reconstituted Complex Homo sapiens 5 CETN1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 WIPF2 147179 Co-fractionation Homo sapiens 7 RAD23B 5887 Reconstituted Complex Homo sapiens 8 UBE3A 7337 Affinity Capture-MS Homo sapiens 9 IKBKG 8517 Affinity Capture-Western Homo sapiens 10 SRP19 6728 Co-fractionation Homo sapiens 11 TRAF6 7189 Affinity Capture-Western Homo sapiens 12 CUL3 8452 Affinity Capture-MS Homo sapiens 13 TOMM34 10953 Co-fractionation Homo sapiens 14 PSMC4 5704 Affinity Capture-MS Homo sapiens 15 UBLCP1   Co-fractionation Homo sapiens 16 LRRK2 120892 Reconstituted Complex Homo sapiens 17 MAP3K1 4214 Biochemical Activity Homo sapiens 18 PSMD6 9861 Affinity Capture-Western Homo sapiens 19 APP 351 Reconstituted Complex Homo sapiens 20 SMURF1 57154 Biochemical Activity Homo sapiens 21 MYH11 4629 Affinity Capture-MS Homo sapiens View the network

Pathways in which ZFAND5 is involved

No pathways found