Gene description for MYH11
Gene name myosin, heavy chain 11, smooth muscle
Gene symbol MYH11
Other names/aliases AAT4
FAA4
SMHC
SMMHC
Species Homo sapiens
 Database cross references - MYH11
ExoCarta ExoCarta_4629
Vesiclepedia VP_4629
Entrez Gene 4629
HGNC 7569
MIM 160745
UniProt P35749  
 MYH11 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
 Gene ontology annotations for MYH11
Molecular Function
    microfilament motor activity GO:0000146 IBA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    structural constituent of muscle GO:0008307 IMP
    actin filament binding GO:0051015 IBA
Biological Process
    smooth muscle contraction GO:0006939 ISS
    skeletal muscle myosin thick filament assembly GO:0030241 ISS
    actomyosin structure organization GO:0031032 IBA
    elastic fiber assembly GO:0048251 IMP
    cardiac muscle cell development GO:0055013 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    muscle myosin complex GO:0005859 TAS
    myosin II complex GO:0016460 IBA
    myosin filament GO:0032982 IBA
    melanosome GO:0042470 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MYH11 in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for MYH11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 EIF3A 8661
Co-fractionation Homo sapiens
3 SLFN11 91607
Proximity Label-MS Homo sapiens
4 GNPDA2 132789
Affinity Capture-MS Homo sapiens
5 LGR4 55366
Affinity Capture-MS Homo sapiens
6 SHC1 6464
Affinity Capture-MS Homo sapiens
7 ARPC5L 81873
Affinity Capture-MS Homo sapiens
8 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
9 SP1  
Affinity Capture-MS Homo sapiens
10 FBXO25  
Affinity Capture-MS Homo sapiens
11 APC  
Two-hybrid Homo sapiens
12 GDF9 2661
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MYOC 4653
Affinity Capture-MS Homo sapiens
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
15 C4BPA 722
Affinity Capture-MS Homo sapiens
16 PARK7 11315
Affinity Capture-MS Homo sapiens
17 PDHA1 5160
Affinity Capture-MS Homo sapiens
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 RWDD2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 DSTN 11034
Co-fractionation Homo sapiens
21 DYRK1A 1859
Affinity Capture-MS Homo sapiens
22 KIF23 9493
Affinity Capture-MS Homo sapiens
23 ACTR3 10096
Affinity Capture-MS Homo sapiens
24 TBC1D31  
Affinity Capture-MS Homo sapiens
25 PLBD2 196463
Affinity Capture-MS Homo sapiens
26 RAD51  
Affinity Capture-MS Homo sapiens
27 YWHAH 7533
Affinity Capture-MS Homo sapiens
28 MPP1 4354
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SFN 2810
Affinity Capture-MS Homo sapiens
30 TFCP2 7024
Affinity Capture-MS Homo sapiens
31 SOD1 6647
Affinity Capture-MS Homo sapiens
32 KIF14 9928
Affinity Capture-MS Homo sapiens
33 MED4 29079
Affinity Capture-MS Homo sapiens
34 CHMP4C 92421
Affinity Capture-MS Homo sapiens
35 ACTA2 59
Co-fractionation Homo sapiens
36 FAM96A  
Affinity Capture-MS Homo sapiens
37 COX15 1355
Affinity Capture-MS Homo sapiens
38 RBX1 9978
Affinity Capture-MS Homo sapiens
39 CLU 1191
Cross-Linking-MS (XL-MS) Homo sapiens
40 SLC9A8 23315
Affinity Capture-MS Homo sapiens
41 POC1A  
Affinity Capture-MS Homo sapiens
42 OVCA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CHMP4B 128866
Affinity Capture-MS Homo sapiens
44 YAP1 10413
Affinity Capture-MS Homo sapiens
45 MYH9 4627
Cross-Linking-MS (XL-MS) Homo sapiens
46 ASTE1  
Affinity Capture-MS Homo sapiens
47 YWHAQ 10971
Affinity Capture-MS Homo sapiens
48 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
49 MYH10 4628
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
50 ARPC5 10092
Affinity Capture-MS Homo sapiens
51 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
52 H2AFY 9555
Cross-Linking-MS (XL-MS) Homo sapiens
53 DNM1L 10059
Affinity Capture-MS Homo sapiens
54 DAXX  
Two-hybrid Homo sapiens
55 YWHAE 7531
Affinity Capture-MS Homo sapiens
56 ANLN 54443
Affinity Capture-MS Homo sapiens
57 ZFAND5 7763
Affinity Capture-MS Homo sapiens
58 LRRC59 55379
Affinity Capture-MS Homo sapiens
59 ECT2 1894
Affinity Capture-MS Homo sapiens
60 TMEM189  
Affinity Capture-MS Homo sapiens
61 HYPK 25764
Cross-Linking-MS (XL-MS) Homo sapiens
62 SLX1B  
Affinity Capture-MS Homo sapiens
63 MYO5A 4644
Co-fractionation Homo sapiens
64 AURKB 9212
Affinity Capture-MS Homo sapiens
65 SOAT1 6646
Affinity Capture-MS Homo sapiens
66 BMPR2 659
Affinity Capture-MS Homo sapiens
67 MYC  
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Affinity Capture-MS Homo sapiens
69 DLST 1743
Affinity Capture-MS Homo sapiens
70 CGN  
Affinity Capture-MS Homo sapiens
71 ACTB 60
Co-fractionation Homo sapiens
72 DDRGK1 65992
Affinity Capture-MS Homo sapiens
73 UBXN2A  
Affinity Capture-MS Homo sapiens
74 VDAC1 7416
Affinity Capture-MS Homo sapiens
75 SIK2  
Affinity Capture-MS Homo sapiens
76 GRB2 2885
Two-hybrid Homo sapiens
77 RALBP1 10928
Affinity Capture-MS Homo sapiens
78 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
79 MYL6 4637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 HIST1H2AC 8334
Cross-Linking-MS (XL-MS) Homo sapiens
81 SVIL 6840
Affinity Capture-MS Homo sapiens
82 ZNF746  
Affinity Capture-MS Homo sapiens
83 RPA1 6117
Affinity Capture-MS Homo sapiens
84 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
85 SECISBP2L  
Cross-Linking-MS (XL-MS) Homo sapiens
86 ATAD3A 55210
Cross-Linking-MS (XL-MS) Homo sapiens
87 COQ2  
Affinity Capture-MS Homo sapiens
88 INF2 64423
Affinity Capture-MS Homo sapiens
89 FLNA 2316
Affinity Capture-MS Homo sapiens
90 YWHAZ 7534
Cross-Linking-MS (XL-MS) Homo sapiens
91 MYO1E 4643
Co-fractionation Homo sapiens
92 RPL23A 6147
Cross-Linking-MS (XL-MS) Homo sapiens
93 B3GNT2 10678
Affinity Capture-MS Homo sapiens
94 RPA2 6118
Affinity Capture-MS Homo sapiens
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