Gene description for MAFK
Gene name v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog K
Gene symbol MAFK
Other names/aliases NFE2U
P18
Species Homo sapiens
 Database cross references - MAFK
ExoCarta ExoCarta_7975
Vesiclepedia VP_7975
Entrez Gene 7975
HGNC 6782
MIM 600197
UniProt O60675  
 MAFK identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MAFK
Molecular Function
    transcription cis-regulatory region binding GO:0000976 ISS
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    DNA-binding transcription factor activity GO:0003700 ISS
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    RNA polymerase II transcription regulator complex GO:0090575 ISS
 Experiment description of studies that identified MAFK in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MAFK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 DNMT3L  
Reconstituted Complex Homo sapiens
3 CEP44  
Affinity Capture-MS Homo sapiens
4 HOXD12  
Reconstituted Complex Homo sapiens
5 HDAC2 3066
Affinity Capture-Western Homo sapiens
6 PARP1 142
Affinity Capture-MS Homo sapiens
7 FANCD2  
Affinity Capture-MS Homo sapiens
8 TP53 7157
Affinity Capture-Western Homo sapiens
9 NFE2L1  
Affinity Capture-MS Homo sapiens
10 ZBTB24  
Affinity Capture-MS Homo sapiens
11 RELA 5970
Affinity Capture-Western Homo sapiens
12 CUL3 8452
Affinity Capture-MS Homo sapiens
13 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
14 CALCOCO1 57658
Affinity Capture-MS Homo sapiens
15 HDAC1 3065
Affinity Capture-Western Homo sapiens
16 MAFG  
Affinity Capture-MS Homo sapiens
17 Bach1 12013
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
18 BACH1 571
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
19 CREBBP  
Affinity Capture-Western Homo sapiens
20 HDAC3 8841
Affinity Capture-Western Homo sapiens
21 SMAD3 4088
Affinity Capture-Western Homo sapiens
22 NFE2L2 4780
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
23 KAT6A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 NFE2L3 9603
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CCDC6 8030
Affinity Capture-MS Homo sapiens
26 NFATC2  
Affinity Capture-MS Homo sapiens
27 CEBPA  
Proximity Label-MS Homo sapiens
28 BACH2 60468
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
29 HIF1A 3091
Reconstituted Complex Homo sapiens
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