Gene description for PAAF1
Gene name proteasomal ATPase-associated factor 1
Gene symbol PAAF1
Other names/aliases PAAF
Rpn14
WDR71
Species Homo sapiens
 Database cross references - PAAF1
ExoCarta ExoCarta_80227
Vesiclepedia VP_80227
Entrez Gene 80227
HGNC 25687
UniProt Q9BRP4  
 PAAF1 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PAAF1
Molecular Function
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IBA
Biological Process
    transcription initiation at RNA polymerase II promoter GO:0006367 IBA
Subcellular Localization
    SAGA complex GO:0000124 IBA
    proteasome complex GO:0000502 IDA
    transcription factor TFIID complex GO:0005669 IBA
 Experiment description of studies that identified PAAF1 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PAAF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 PSMB6 5694
Co-fractionation Homo sapiens
3 PSMD5 5711
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
4 PSMA3 5684
Co-fractionation Homo sapiens
5 SHFM1 7979
Affinity Capture-MS Homo sapiens
6 PPP4R2  
Co-fractionation Homo sapiens
7 TK1 7083
Two-hybrid Homo sapiens
8 PSMD6 9861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ZFAND2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BAG1 573
Affinity Capture-MS Homo sapiens
11 PSMA4 5685
Co-fractionation Homo sapiens
12 PSMD13 5719
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PSMD8 5714
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 POLR2E 5434
Co-fractionation Homo sapiens
15 ANXA7 310
Two-hybrid Homo sapiens
16 PSMB8 5696
Co-fractionation Homo sapiens
17 PSMA2 5683
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
18 ALDH16A1 126133
Affinity Capture-MS Homo sapiens
19 PSMB4 5692
Co-fractionation Homo sapiens
20 FAM46B  
Affinity Capture-MS Homo sapiens
21 FAM21A 387680
Co-fractionation Homo sapiens
22 PSMD4 5710
Affinity Capture-MS Homo sapiens
23 FAM109A  
Affinity Capture-MS Homo sapiens
24 HDAC1 3065
Co-fractionation Homo sapiens
25 PSMD11 5717
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
26 PSMD10 5716
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
27 PTPN1 5770
Co-fractionation Homo sapiens
28 CCDC92  
Affinity Capture-MS Homo sapiens
29 PSMD7 5713
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 VASP 7408
Co-fractionation Homo sapiens
31 PSMC1 5700
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PSMA1 5682
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
33 PSMD14 10213
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
34 PSMD2 5708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CAPZB 832
Co-fractionation Homo sapiens
36 RPA3 6119
Proximity Label-MS Homo sapiens
37 PSMB3 5691
Co-fractionation Homo sapiens
38 UBLCP1  
Affinity Capture-MS Homo sapiens
39 SUPT6H 6830
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 PSMA7 5688
Co-fractionation Homo sapiens
41 MON2 23041
Co-fractionation Homo sapiens
42 GPHN 10243
Co-fractionation Homo sapiens
43 FADS1 3992
Affinity Capture-MS Homo sapiens
44 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
45 PSMB5 5693
Co-fractionation Homo sapiens
46 PSMD9 5715
Affinity Capture-MS Homo sapiens
47 C7orf76  
Affinity Capture-MS Homo sapiens
48 ADRM1 11047
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PSMA5 5686
Co-fractionation Homo sapiens
50 PSMC6 5706
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
51 PSMD1 5707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DERA 51071
Affinity Capture-MS Homo sapiens
53 PSMC4 5704
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
54 PSMC2 5701
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
55 CCDC6 8030
Co-fractionation Homo sapiens
56 Psmd6 66413
Affinity Capture-MS Mus musculus
57 PSMB1 5689
Co-fractionation Homo sapiens
58 PSMD12 5718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
59 ELP2 55250
Co-fractionation Homo sapiens
60 PSMD3 5709
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
61 UCHL5 51377
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 PSMC3 5702
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here