Gene description for EIF2B3
Gene name eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa
Gene symbol EIF2B3
Other names/aliases EIF-2B
EIF2Bgamma
Species Homo sapiens
 Database cross references - EIF2B3
ExoCarta ExoCarta_8891
Vesiclepedia VP_8891
Entrez Gene 8891
HGNC 3259
MIM 606273
UniProt Q9NR50  
 EIF2B3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for EIF2B3
Molecular Function
    translation initiation factor activity GO:0003743 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    protein binding GO:0005515 IPI
    translation factor activity, RNA binding GO:0008135 IDA
Biological Process
    cytoplasmic translational initiation GO:0002183 IBA
    cytoplasmic translational initiation GO:0002183 IDA
    translational initiation GO:0006413 IDA
    response to heat GO:0009408 ISS
    response to heat GO:0009408 TAS
    response to glucose GO:0009749 ISS
    oligodendrocyte development GO:0014003 IMP
    response to peptide hormone GO:0043434 ISS
    T cell receptor signaling pathway GO:0050852 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    eukaryotic translation initiation factor 2B complex GO:0005851 IBA
    eukaryotic translation initiation factor 2B complex GO:0005851 IDA
    guanyl-nucleotide exchange factor complex GO:0032045 IBA
 Experiment description of studies that identified EIF2B3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2B3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 IGF2BP1 10642
Cross-Linking-MS (XL-MS) Homo sapiens
3 Cdk1 12534
Affinity Capture-MS Mus musculus
4 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 EIF3C 8663
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
6 RPA2 6118
Proximity Label-MS Homo sapiens
7 CD4 920
Affinity Capture-MS Homo sapiens
8 EIF2B2 8892
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
9 ABCF1 23
Co-fractionation Homo sapiens
10 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 C9orf78 51759
Affinity Capture-MS Homo sapiens
12 PNKD  
Affinity Capture-MS Homo sapiens
13 ILF2 3608
Co-fractionation Homo sapiens
14 KIAA1429 25962
Affinity Capture-MS Homo sapiens
15 DIMT1 27292
Co-fractionation Homo sapiens
16 UXS1 80146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 NDUFB8 4714
Two-hybrid Homo sapiens
18 CLEC2B  
Affinity Capture-MS Homo sapiens
19 DDRGK1 65992
Affinity Capture-MS Homo sapiens
20 QARS 5859
Co-fractionation Homo sapiens
21 SYT2  
Affinity Capture-MS Homo sapiens
22 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 EXD2  
Proximity Label-MS Homo sapiens
24 TULP3 7289
Affinity Capture-MS Homo sapiens
25 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
26 ZWINT  
Affinity Capture-MS Homo sapiens
27 CA14 23632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NMT1 4836
Co-fractionation Homo sapiens
29 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 GPR17 2840
Affinity Capture-MS Homo sapiens
32 EIF2S3 1968
Affinity Capture-MS Homo sapiens
33 EDC4 23644
Co-fractionation Homo sapiens
34 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 MRE11A 4361
Co-fractionation Homo sapiens
37 CD74 972
Affinity Capture-MS Homo sapiens
38 PARK2  
Affinity Capture-MS Homo sapiens
39 TMEM55B 90809
Affinity Capture-MS Homo sapiens
40 PLD1 5337
Affinity Capture-MS Homo sapiens
41 GCH1  
Affinity Capture-MS Homo sapiens
42 COMTD1 118881
Affinity Capture-MS Homo sapiens
43 TACSTD2 4070
Affinity Capture-MS Homo sapiens
44 RFC4 5984
Co-fractionation Homo sapiens
45 DDX1 1653
Co-fractionation Homo sapiens
46 DHX9 1660
Co-fractionation Homo sapiens
47 C3orf18  
Affinity Capture-MS Homo sapiens
48 EIF2B4 8890
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
49 CACNG4  
Affinity Capture-MS Homo sapiens
50 P2RX4 5025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ECH1 1891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 FGF14  
Affinity Capture-MS Homo sapiens
54 BTNL9  
Affinity Capture-MS Homo sapiens
55 XPO1 7514
Affinity Capture-MS Homo sapiens
56 FZD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CUL3 8452
Affinity Capture-MS Homo sapiens
58 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 FBXO6 26270
Affinity Capture-MS Homo sapiens
60 EIF2B5 8893
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
61 CDC42 998
Affinity Capture-MS Homo sapiens
62 CD226 10666
Affinity Capture-MS Homo sapiens
63 SMDT1  
Affinity Capture-MS Homo sapiens
64 HTR2C  
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 DCC  
Affinity Capture-MS Homo sapiens
67 SCN2B  
Affinity Capture-MS Homo sapiens
68 FLII 2314
Co-fractionation Homo sapiens
69 PVRL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 ENTPD2 954
Affinity Capture-MS Homo sapiens
71 STC2 8614
Two-hybrid Homo sapiens
72 EIF2B1 1967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
73 TMEM55A 55529
Affinity Capture-MS Homo sapiens
74 NELFB 25920
Co-fractionation Homo sapiens
75 PTAFR  
Affinity Capture-MS Homo sapiens
76 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
77 FKBP3 2287
Co-fractionation Homo sapiens
78 BTC  
Affinity Capture-MS Homo sapiens
79 TNFSF14  
Affinity Capture-MS Homo sapiens
80 HNRNPM 4670
Co-fractionation Homo sapiens
81 EFNB1 1947
Affinity Capture-MS Homo sapiens
82 G3BP1 10146
Co-fractionation Homo sapiens
83 OPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 IGF2BP3 10643
Co-fractionation Homo sapiens
86 SPN 6693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 SEC62 7095
Affinity Capture-MS Homo sapiens
88 RTCB 51493
Co-fractionation Homo sapiens
89 YBX3 8531
Co-fractionation Homo sapiens
90 C14orf166 51637
Co-fractionation Homo sapiens
91 TPST2 8459
Affinity Capture-MS Homo sapiens
92 SERBP1 26135
Affinity Capture-MS Homo sapiens
93 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
94 HNRNPU 3192
Co-fractionation Homo sapiens
95 PLD6  
Affinity Capture-MS Homo sapiens
96 SYT1 6857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 EIF5 1983
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here