Gene description for CDK5R2
Gene name cyclin-dependent kinase 5, regulatory subunit 2 (p39)
Gene symbol CDK5R2
Other names/aliases NCK5AI
P39
p39nck5ai
Species Homo sapiens
 Database cross references - CDK5R2
ExoCarta ExoCarta_8941
Vesiclepedia VP_8941
Entrez Gene 8941
HGNC 1776
MIM 603764
UniProt Q13319  
 CDK5R2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CDK5R2
Molecular Function
    actin binding GO:0003779 ISS
    lipid binding GO:0008289 IEA
    protein kinase binding GO:0019901 IBA
    cyclin-dependent protein serine/threonine kinase activator activity GO:0061575 IBA
    cyclin-dependent protein serine/threonine kinase activator activity GO:0061575 ISS
    cyclin-dependent protein serine/threonine kinase activator activity GO:0061575 TAS
Biological Process
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 TAS
    neuron migration GO:0001764 IEA
    axon guidance GO:0007411 IBA
    brain development GO:0007420 IBA
    cerebellum development GO:0021549 IEA
    superior olivary nucleus maturation GO:0021722 IEA
    hippocampus development GO:0021766 IEA
    layer formation in cerebral cortex GO:0021819 IEA
    positive regulation of calcium ion-dependent exocytosis GO:0045956 IEA
Subcellular Localization
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 ISS
    protein kinase 5 complex GO:0016533 ISS
    growth cone GO:0030426 IBA
    growth cone GO:0030426 ISS
    neuron projection GO:0043005 ISS
    presynapse GO:0098793 IEA
 Experiment description of studies that identified CDK5R2 in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CDK5R2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2BF 440689
Cross-Linking-MS (XL-MS) Homo sapiens
2 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
3 HIST2H2BE 8349
Cross-Linking-MS (XL-MS) Homo sapiens
4 ACTN1 87
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
5 HIST1H2BJ 8970
Cross-Linking-MS (XL-MS) Homo sapiens
6 TOP2A 7153
Cross-Linking-MS (XL-MS) Homo sapiens
7 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
8 ADAMTS12 81792
Cross-Linking-MS (XL-MS) Homo sapiens
9 HIST1H2BB 3018
Cross-Linking-MS (XL-MS) Homo sapiens
10 HIST3H2BB 128312
Cross-Linking-MS (XL-MS) Homo sapiens
11 EEF1A1 1915
Cross-Linking-MS (XL-MS) Homo sapiens
12 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
13 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
14 TEX35  
Cross-Linking-MS (XL-MS) Homo sapiens
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