Gene description for MCFD2
Gene name multiple coagulation factor deficiency 2
Gene symbol MCFD2
Other names/aliases F5F8D
F5F8D2
LMAN1IP
SDNSF
Species Homo sapiens
 Database cross references - MCFD2
ExoCarta ExoCarta_90411
Vesiclepedia VP_90411
Entrez Gene 90411
HGNC 18451
MIM 607788
UniProt Q8NI22  
 MCFD2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for MCFD2
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IEA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 TAS
 Experiment description of studies that identified MCFD2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MCFD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Co-fractionation Homo sapiens
2 LARS 51520
Co-fractionation Homo sapiens
3 HKDC1 80201
Affinity Capture-MS Homo sapiens
4 COPG2 26958
Affinity Capture-MS Homo sapiens
5 MCM3 4172
Co-fractionation Homo sapiens
6 HSPA5 3309
Affinity Capture-MS Homo sapiens
7 AGL 178
Co-fractionation Homo sapiens
8 HIBADH 11112
Co-fractionation Homo sapiens
9 COPZ1 22818
Affinity Capture-MS Homo sapiens
10 SLC35A1 10559
Two-hybrid Homo sapiens
11 PSEN1 5663
Co-fractionation Homo sapiens
12 IDH1 3417
Co-fractionation Homo sapiens
13 PSMD12 5718
Co-fractionation Homo sapiens
14 KLHL9 55958
Affinity Capture-MS Homo sapiens
15 PSMD6 9861
Co-fractionation Homo sapiens
16 KIF20A 10112
Affinity Capture-MS Homo sapiens
17 ENOPH1 58478
Co-fractionation Homo sapiens
18 PNLIPRP1  
Two-hybrid Homo sapiens
19 EIF6 3692
Co-fractionation Homo sapiens
20 P2RY6  
Two-hybrid Homo sapiens
21 SEC13 6396
Affinity Capture-MS Homo sapiens
22 PSMD11 5717
Co-fractionation Homo sapiens
23 PFKP 5214
Co-fractionation Homo sapiens
24 TXNRD2 10587
Co-fractionation Homo sapiens
25 PFKM 5213
Co-fractionation Homo sapiens
26 SEPHS1 22929
Co-fractionation Homo sapiens
27 PACSIN2 11252
Co-fractionation Homo sapiens
28 SKP1 6500
Co-fractionation Homo sapiens
29 LMAN1 3998
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
30 CAPZA1 829
Co-fractionation Homo sapiens
31 DCTN2 10540
Co-fractionation Homo sapiens
32 PSMD4 5710
Co-fractionation Homo sapiens
33 RUVBL2 10856
Co-fractionation Homo sapiens
34 POLR2D  
Co-fractionation Homo sapiens
35 ANXA6 309
Co-fractionation Homo sapiens
36 XRN2 22803
Co-fractionation Homo sapiens
37 COPE 11316
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
38 YIPF5 81555
Affinity Capture-MS Homo sapiens
39 COPA 1314
Affinity Capture-MS Homo sapiens
40 DDX58 23586
Affinity Capture-RNA Homo sapiens
41 PSMD7 5713
Co-fractionation Homo sapiens
42 ECHS1 1892
Co-fractionation Homo sapiens
43 APP 351
Reconstituted Complex Homo sapiens
44 KLHL13 90293
Affinity Capture-MS Homo sapiens
45 PSMD14 10213
Co-fractionation Homo sapiens
46 GPX1 2876
Co-fractionation Homo sapiens
47 CHCHD4  
Co-fractionation Homo sapiens
48 BCL2L13 23786
Co-fractionation Homo sapiens
49 LAGE3  
Co-fractionation Homo sapiens
50 YWHAE 7531
Co-fractionation Homo sapiens
51 NLRP3  
Co-fractionation Homo sapiens
52 ACTN4 81
Co-fractionation Homo sapiens
53 FDPS 2224
Co-fractionation Homo sapiens
54 VARS 7407
Co-fractionation Homo sapiens
55 CMTM5  
Two-hybrid Homo sapiens
56 TMOD3 29766
Co-fractionation Homo sapiens
57 GOT1 2805
Co-fractionation Homo sapiens
58 AADAT  
Co-fractionation Homo sapiens
59 DSTN 11034
Co-fractionation Homo sapiens
60 YWHAQ 10971
Co-fractionation Homo sapiens
61 SNRPD1 6632
Co-fractionation Homo sapiens
62 SGTA 6449
Co-fractionation Homo sapiens
63 UBXN6 80700
Affinity Capture-MS Homo sapiens
64 YWHAG 7532
Co-fractionation Homo sapiens
65 PSMC4 5704
Co-fractionation Homo sapiens
66 PSMC2 5701
Co-fractionation Homo sapiens
67 ACOX1 51
Co-fractionation Homo sapiens
68 SND1 27044
Co-fractionation Homo sapiens
69 CAPZB 832
Co-fractionation Homo sapiens
70 SYS1  
Two-hybrid Homo sapiens
71 IL1RL1 9173
Two-hybrid Homo sapiens
72 CARS 833
Co-fractionation Homo sapiens
73 PSMD3 5709
Co-fractionation Homo sapiens
74 AIMP1 9255
Co-fractionation Homo sapiens
75 GSR 2936
Co-fractionation Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here