Gene description for GCNT3
Gene name glucosaminyl (N-acetyl) transferase 3, mucin type
Gene symbol GCNT3
Other names/aliases C2/4GnT
C24GNT
C2GNT2
C2GNTM
GNTM
Species Homo sapiens
 Database cross references - GCNT3
ExoCarta ExoCarta_9245
Vesiclepedia VP_9245
Entrez Gene 9245
HGNC 4205
MIM 606836
UniProt O95395  
 GCNT3 identified in sEVs derived from the following tissue/cell type
Saliva 19199708    
 Gene ontology annotations for GCNT3
Molecular Function
    beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity GO:0003829 TAS
    N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity GO:0008109 IEA
    acetylglucosaminyltransferase activity GO:0008375 IBA
    acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity GO:0047225 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 NAS
    protein O-linked glycosylation GO:0006493 TAS
    O-glycan processing GO:0016266 TAS
    tissue morphogenesis GO:0048729 IEA
    intestinal absorption GO:0050892 IEA
    kidney morphogenesis GO:0060993 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GCNT3 in sEVs
1
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for GCNT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HERC1 8925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ACTA2 59
Affinity Capture-MS Homo sapiens
3 CUL4B 8450
Affinity Capture-MS Homo sapiens
4 KLHDC2  
Affinity Capture-MS Homo sapiens
5 RSPRY1 89970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 BCKDK 10295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GLRA3  
Affinity Capture-MS Homo sapiens
8 SNUPN 10073
Two-hybrid Homo sapiens
9 SORL1 6653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 DHRSX 207063
Two-hybrid Homo sapiens
11 CXCR4 7852
Affinity Capture-MS Homo sapiens
12 CUL2 8453
Affinity Capture-MS Homo sapiens
13 RBM18  
Two-hybrid Homo sapiens
14 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
15 TBC1D15 64786
Affinity Capture-MS Homo sapiens
16 ATP11C 286410
Affinity Capture-MS Homo sapiens
17 FEM1B 10116
Affinity Capture-MS Homo sapiens
18 CASK 8573
Two-hybrid Homo sapiens
19 APPBP2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here