Gene description for CYTH2
Gene name cytohesin 2
Gene symbol CYTH2
Other names/aliases ARNO
CTS18
CTS18.1
PSCD2
PSCD2L
SEC7L
Sec7p-L
Sec7p-like
Species Homo sapiens
 Database cross references - CYTH2
ExoCarta ExoCarta_9266
Vesiclepedia VP_9266
Entrez Gene 9266
HGNC 9502
MIM 602488
UniProt Q99418  
 CYTH2 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CYTH2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
    inositol 1,4,5 trisphosphate binding GO:0070679 ISS
Biological Process
    endocytosis GO:0006897 TAS
    actin cytoskeleton organization GO:0030036 TAS
    regulation of ARF protein signal transduction GO:0032012 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    adherens junction GO:0005912 IEA
    bicellular tight junction GO:0005923 IEA
    membrane GO:0016020 TAS
    growth cone GO:0030426 IEA
 Experiment description of studies that identified CYTH2 in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CYTH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 SFN 2810
Affinity Capture-MS Homo sapiens
3 ARRB2 409
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 CNKSR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 Ipcef1  
Two-hybrid Rattus norvegicus
6 ATPIF1 93974
Affinity Capture-MS Homo sapiens
7 STRIP2  
Affinity Capture-MS Homo sapiens
8 VAC14 55697
Affinity Capture-MS Homo sapiens
9 C1orf106  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CCDC120  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 APC  
Two-hybrid Homo sapiens
12 GNAQ 2776
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 CYTH1 9267
Affinity Capture-MS Homo sapiens
14 ARF6 382
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 DDX58 23586
Affinity Capture-RNA Homo sapiens
16 FOSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 IPCEF1 26034
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 KCNE3  
Affinity Capture-MS Homo sapiens
20 MAPK6  
Two-hybrid Homo sapiens
21 TAX1BP3 30851
Affinity Capture-MS Homo sapiens
22 MTMR7  
Affinity Capture-MS Homo sapiens
23 TRIM23 373
Reconstituted Complex Homo sapiens
24 ARRB1 408
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
25 MYD88 4615
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 SSSCA1 10534
Affinity Capture-MS Homo sapiens
27 ARF1 375
Biochemical Activity Homo sapiens
28 PIK3CA 5290
PCA Homo sapiens
29 PYCRL 65263
Affinity Capture-MS Homo sapiens
30 ADORA2A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
31 CCDC40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PNPLA2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
33 CYTIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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