Gene description for SLC38A5
Gene name solute carrier family 38, member 5
Gene symbol SLC38A5
Other names/aliases JM24
SN2
SNAT5
pp7194
Species Homo sapiens
 Database cross references - SLC38A5
ExoCarta ExoCarta_92745
Vesiclepedia VP_92745
Entrez Gene 92745
HGNC 18070
MIM 300649
UniProt Q8WUX1  
 SLC38A5 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SLC38A5
Molecular Function
    L-histidine transmembrane transporter activity GO:0005290 IMP
    L-histidine transmembrane transporter activity GO:0005290 ISS
    amino acid transmembrane transporter activity GO:0015171 TAS
    L-asparagine transmembrane transporter activity GO:0015182 IMP
    L-glutamine transmembrane transporter activity GO:0015186 IBA
    L-glutamine transmembrane transporter activity GO:0015186 IMP
    L-glutamine transmembrane transporter activity GO:0015186 ISS
    glycine transmembrane transporter activity GO:0015187 IBA
    glycine transmembrane transporter activity GO:0015187 IMP
    L-serine transmembrane transporter activity GO:0015194 IMP
    L-serine transmembrane transporter activity GO:0015194 ISS
    alanine transmembrane transporter activity GO:0022858 IMP
    L-glutamine, sodium:proton antiporter activity GO:0140830 ISS
    neutral amino acid, sodium:proton antiporter activity GO:0140893 IDA
Biological Process
    amino acid transport GO:0006865 TAS
    asparagine transport GO:0006867 IEA
    glutamine transport GO:0006868 IBA
    glutamine transport GO:0006868 IMP
    glutamine transport GO:0006868 ISS
    neutral amino acid transport GO:0015804 IDA
    glycine transport GO:0015816 IBA
    glycine transport GO:0015816 IMP
    L-serine transport GO:0015825 ISS
    serine transport GO:0032329 IBA
    serine transport GO:0032329 IMP
    amino acid export across plasma membrane GO:0032973 ISS
    sodium ion transmembrane transport GO:0035725 IEA
    L-histidine transmembrane transport GO:0089709 IBA
    L-histidine transmembrane transport GO:0089709 IMP
    L-histidine transmembrane transport GO:0089709 ISS
    amino acid import across plasma membrane GO:0089718 ISS
    transport across blood-brain barrier GO:0150104 NAS
    asparagine transmembrane transport GO:1903713 IMP
    L-glutamine import across plasma membrane GO:1903803 ISS
    L-serine import across plasma membrane GO:1903812 ISS
    L-alanine transmembrane transport GO:1904557 IMP
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified SLC38A5 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC38A5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LGALS12  
Affinity Capture-MS Homo sapiens
2 LGALS9 3965
Affinity Capture-MS Homo sapiens
3 TMEM63B 55362
Affinity Capture-MS Homo sapiens
4 CLEC2D  
Affinity Capture-MS Homo sapiens
5 LGALS3 3958
Affinity Capture-MS Homo sapiens
6 PRKAR1A 5573
Co-fractionation Homo sapiens
7 TSPAN15 23555
Affinity Capture-MS Homo sapiens
8 UBC 7316
Reconstituted Complex Homo sapiens
9 KLRB1  
Affinity Capture-MS Homo sapiens
10 YIPF5 81555
Affinity Capture-MS Homo sapiens
11 C17orf49 124944
Affinity Capture-MS Homo sapiens
12 CD27  
Affinity Capture-MS Homo sapiens
13 ARMC6 93436
Affinity Capture-MS Homo sapiens
14 Kcnk1  
Affinity Capture-MS Mus musculus
15 VAMP5 10791
Affinity Capture-MS Homo sapiens
16 SPPL2B 56928
Affinity Capture-MS Homo sapiens
17 HLA-B 3106
Affinity Capture-MS Homo sapiens
18 TNFRSF10C  
Affinity Capture-MS Homo sapiens
19 IL27RA 9466
Affinity Capture-MS Homo sapiens
20 CACNG7  
Affinity Capture-MS Homo sapiens
21 Tnpo1 238799
Affinity Capture-MS Mus musculus
22 ENTPD2 954
Affinity Capture-MS Homo sapiens
23 RAB5C 5878
Affinity Capture-MS Homo sapiens
24 ATP2A3 489
Affinity Capture-MS Homo sapiens
25 Smn1 20595
Affinity Capture-MS Mus musculus
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