Gene description for ORMDL3
Gene name ORMDL sphingolipid biosynthesis regulator 3
Gene symbol ORMDL3
Other names/aliases -
Species Homo sapiens
 Database cross references - ORMDL3
ExoCarta ExoCarta_94103
Vesiclepedia VP_94103
Entrez Gene 94103
HGNC 16038
MIM 610075
UniProt Q8N138  
 ORMDL3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ORMDL3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of B cell apoptotic process GO:0002903 IMP
    ceramide metabolic process GO:0006672 IBA
    ceramide metabolic process GO:0006672 IMP
    sphingomyelin biosynthetic process GO:0006686 IEA
    regulation of smooth muscle contraction GO:0006940 IEA
    positive regulation of autophagy GO:0010508 IDA
    positive regulation of autophagy GO:0010508 IMP
    sphingolipid biosynthetic process GO:0030148 IBA
    myelination GO:0042552 IEA
    motor behavior GO:0061744 IEA
    intracellular sphingolipid homeostasis GO:0090156 IBA
    negative regulation of ceramide biosynthetic process GO:1900060 IMP
    positive regulation of protein localization to nucleus GO:1900182 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 TAS
    serine palmitoyltransferase complex GO:0017059 IBA
    serine palmitoyltransferase complex GO:0017059 IDA
    secretory granule membrane GO:0030667 TAS
    specific granule membrane GO:0035579 TAS
 Experiment description of studies that identified ORMDL3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ORMDL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 MAGEA8  
Affinity Capture-MS Homo sapiens
3 SLC2A12 154091
Affinity Capture-MS Homo sapiens
4 C10orf67  
Two-hybrid Homo sapiens
5 SV2A 9900
Affinity Capture-MS Homo sapiens
6 ATP13A3 79572
Affinity Capture-MS Homo sapiens
7 LEPROTL1 23484
Two-hybrid Homo sapiens
8 ADCK3  
Two-hybrid Homo sapiens
9 TGM3 7053
Affinity Capture-MS Homo sapiens
10 MTIF3  
Two-hybrid Homo sapiens
11 ERGIC3 51614
Two-hybrid Homo sapiens
12 BSCL2  
Affinity Capture-MS Homo sapiens
13 SPTLC1 10558
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 GPR101  
Two-hybrid Homo sapiens
15 EEF1A1 1915
Two-hybrid Homo sapiens
16 APP 351
Reconstituted Complex Homo sapiens
17 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
18 SLC5A6 8884
Affinity Capture-MS Homo sapiens
19 EBP  
Two-hybrid Homo sapiens
20 REEP2  
Two-hybrid Homo sapiens
21 AQP3  
Affinity Capture-MS Homo sapiens
22 SLC22A9  
Affinity Capture-MS Homo sapiens
23 CERS3  
Two-hybrid Homo sapiens
24 PDE2A  
Affinity Capture-MS Homo sapiens
25 GPR152  
Two-hybrid Homo sapiens
26 FAM209A 200232
Two-hybrid Homo sapiens
27 SLC18A2  
Affinity Capture-MS Homo sapiens
28 CSNK1D 1453
Affinity Capture-MS Homo sapiens
29 FAM134C 162427
Two-hybrid Homo sapiens
30 HSD17B13  
Two-hybrid Homo sapiens
31 TMEM237 65062
Two-hybrid Homo sapiens
32 WRB 7485
Two-hybrid Homo sapiens
33 SPRY4 81848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SELENBP1 8991
Affinity Capture-MS Homo sapiens
35 SLC10A1  
Two-hybrid Homo sapiens
36 GABBR1  
Affinity Capture-MS Homo sapiens
37 DUSP14 11072
Affinity Capture-MS Homo sapiens
38 KCNK5  
Two-hybrid Homo sapiens
39 CRELD1 78987
Affinity Capture-MS Homo sapiens
40 RNF5  
Two-hybrid Homo sapiens
41 ARL13B 200894
Two-hybrid Homo sapiens
42 SLC1A1 6505
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 SLC22A4 6583
Affinity Capture-MS Homo sapiens
44 SYT2  
Two-hybrid Homo sapiens
45 ELOVL4  
Two-hybrid Homo sapiens
46 LNX1  
Two-hybrid Homo sapiens
47 SLC7A1 6541
Two-hybrid Homo sapiens
48 ROM1  
Two-hybrid Homo sapiens
View the network image/svg+xml



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