Gene description for PCYT1B
Gene name phosphate cytidylyltransferase 1, choline, beta
Gene symbol PCYT1B
Other names/aliases CCTB
CTB
Species Homo sapiens
 Database cross references - PCYT1B
ExoCarta ExoCarta_9468
Vesiclepedia VP_9468
Entrez Gene 9468
HGNC 8755
MIM 300948
UniProt Q9Y5K3  
 PCYT1B identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for PCYT1B
Molecular Function
    choline-phosphate cytidylyltransferase activity GO:0004105 IBA
    choline-phosphate cytidylyltransferase activity GO:0004105 IDA
    protein binding GO:0005515 IPI
    phosphatidylcholine binding GO:0031210 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    ovarian follicle development GO:0001541 IEA
    phosphatidylcholine biosynthetic process GO:0006656 IDA
    CDP-choline pathway GO:0006657 IDA
    spermatogenesis GO:0007283 IEA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified PCYT1B in sEVs
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PCYT1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 ACTA2 59
Co-crystal Structure Homo sapiens
3 ACTB 60
Co-crystal Structure Homo sapiens
4 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 TMEM43 79188
Two-hybrid Homo sapiens
6 KIAA0368 23392
Two-hybrid Homo sapiens
7 ACTA1 58
Co-crystal Structure Homo sapiens
8 PCYT1A 5130
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
9 PAXIP1  
Two-hybrid Homo sapiens
10 PCYT1B 9468
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here