Gene description for KIAA0355
Gene name KIAA0355
Gene symbol KIAA0355
Other names/aliases -
Species Homo sapiens
 Database cross references - KIAA0355
ExoCarta ExoCarta_9710
Vesiclepedia VP_9710
Entrez Gene 9710
HGNC 29016
UniProt O15063  
 KIAA0355 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for KIAA0355
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
    CCR4-NOT complex binding GO:1905762 IBA
    CCR4-NOT complex binding GO:1905762 IDA
Biological Process
    Rac protein signal transduction GO:0016601 IBA
    Rac protein signal transduction GO:0016601 IDA
Subcellular Localization
    P-body GO:0000932 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified KIAA0355 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIAA0355
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB35 11021
Proximity Label-MS Homo sapiens
2 FBXW11  
Affinity Capture-MS Homo sapiens
3 KIF14 9928
Affinity Capture-MS Homo sapiens
4 MYH13 8735
Affinity Capture-MS Homo sapiens
5 CAMKK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 LAMTOR1 55004
Proximity Label-MS Homo sapiens
7 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CPEB1  
Proximity Label-MS Homo sapiens
9 NCKAP1 10787
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TRIM66  
Affinity Capture-MS Homo sapiens
11 PHIP 55023
Affinity Capture-MS Homo sapiens
12 CUL3 8452
Affinity Capture-MS Homo sapiens
13 YWHAZ 7534
Affinity Capture-MS Homo sapiens
14 RB1CC1 9821
Affinity Capture-MS Homo sapiens
15 CLU 1191
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 Wdr5  
Affinity Capture-MS Mus musculus
17 DUSP16  
Affinity Capture-MS Homo sapiens
18 APOD 347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ABI2 10152
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 MUCL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RPA3 6119
Proximity Label-MS Homo sapiens
22 BRD1 23774
Affinity Capture-MS Homo sapiens
23 BICD2 23299
Proximity Label-MS Homo sapiens
24 AMOT 154796
Proximity Label-MS Homo sapiens
25 RHOB 388
Proximity Label-MS Homo sapiens
26 YWHAG 7532
Affinity Capture-MS Homo sapiens
27 AGPS 8540
Proximity Label-MS Homo sapiens
28 STIL  
Proximity Label-MS Homo sapiens
29 ACBD5 91452
Proximity Label-MS Homo sapiens
30 KRBOX4  
Affinity Capture-MS Homo sapiens
31 SP100 6672
Affinity Capture-MS Homo sapiens
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