Gene description for Phactr4
Gene name phosphatase and actin regulator 4
Gene symbol Phactr4
Other names/aliases 3110001B12Rik
AI527228
AW495572
C330013F19Rik
N28169
mKIAA4120
Species Mus musculus
 Database cross references - Phactr4
ExoCarta ExoCarta_100169
Vesiclepedia VP_100169
Entrez Gene 100169
UniProt Q501J7  
 Phactr4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Phactr4
Molecular Function
    actin binding GO:0003779 IBA
    actin binding GO:0003779 IDA
    protein phosphatase inhibitor activity GO:0004864 IEA
    protein phosphatase 1 binding GO:0008157 IDA
    protein phosphatase activator activity GO:0072542 IDA
    protein phosphatase activator activity GO:0072542 IMP
Biological Process
    neural crest cell migration GO:0001755 IBA
    neural crest cell migration GO:0001755 IMP
    neural tube closure GO:0001843 IMP
    Rho protein signal transduction GO:0007266 IMP
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 IMP
    positive regulation of catalytic activity GO:0043085 IDA
    enteric nervous system development GO:0048484 IMP
    regulation of cell cycle GO:0051726 IMP
    closure of optic fissure GO:0061386 IMP
    negative regulation of integrin-mediated signaling pathway GO:2001045 IMP
Subcellular Localization
    cytoplasm GO:0005737 IEA
    lamellipodium GO:0030027 IDA
 Experiment description of studies that identified Phactr4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Phactr4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Phactr4 is involved
No pathways found





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