| Experiment ID |
226 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
GAPDH|CD9|FLOT1
|
Enriched markers |
|
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
pseudogene
|
| Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
| PubMed ID |
26027894
|
| Organism |
Homo sapiens |
| Experiment description |
"Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" |
| Authors |
"Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" |
| Journal name |
Journal of Extracellular Vesicles
|
| Publication year |
2015 |
| Sample |
Endothelial cells |
| Sample name |
HMEC-1 |
| Isolation/purification methods |
Differential ultracentrifugation Sucrose density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA |
| Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting |