Gene description for Numa1
Gene name nuclear mitotic apparatus protein 1
Gene symbol Numa1
Other names/aliases 6720401E04Rik
AA764025
AL022610
AU014979
Species Mus musculus
 Database cross references - Numa1
ExoCarta ExoCarta_101706
Entrez Gene 101706
 Numa1 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Mast cells 17486113    
 Gene ontology annotations for Numa1
Molecular Function
    microtubule binding GO:0008017 ISO
    tubulin binding GO:0015631 ISO
Biological Process
    lung epithelial cell differentiation GO:0060487 IMP
    establishment of mitotic spindle orientation GO:0000132 IMP
Subcellular Localization
    apical part of cell GO:0045177 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    dendrite GO:0030425 ISO
    cell GO:0005623 IMP
    spindle pole GO:0000922 ISO
    neuronal cell body GO:0043025 ISO
    extracellular exosome GO:0070062 ISO
    spindle microtubule GO:0005876 ISO
    nuclear matrix GO:0016363 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Numa1 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Numa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Numa1 is involved
No pathways found





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