Gene description for Numa1
Gene name nuclear mitotic apparatus protein 1
Gene symbol Numa1
Other names/aliases 6720401E04Rik
AA764025
AL022610
AU014979
Species Mus musculus
 Database cross references - Numa1
ExoCarta ExoCarta_101706
Vesiclepedia VP_101706
Entrez Gene 101706
 Numa1 identified in sEVs derived from the following tissue/cell type
Fibroblasts 23260141    
Mast cells 17486113    
 Gene ontology annotations for Numa1
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 ISO
    microtubule binding GO:0008017 ISS
    tubulin binding GO:0015631 ISO
    tubulin binding GO:0015631 ISS
    protein domain specific binding GO:0019904 ISO
    phosphatidylinositol binding GO:0035091 ISO
    phosphatidylinositol binding GO:0035091 ISS
    protein-containing complex binding GO:0044877 ISO
    microtubule plus-end binding GO:0051010 ISO
    microtubule plus-end binding GO:0051010 ISS
    microtubule minus-end binding GO:0051011 ISO
    microtubule minus-end binding GO:0051011 ISS
    dynein complex binding GO:0070840 ISO
    dynein complex binding GO:0070840 ISS
    disordered domain specific binding GO:0097718 IEA
    disordered domain specific binding GO:0097718 ISO
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IBA
    establishment of mitotic spindle orientation GO:0000132 IMP
    establishment of mitotic spindle orientation GO:0000132 ISO
    establishment of mitotic spindle orientation GO:0000132 ISS
    microtubule bundle formation GO:0001578 ISO
    microtubule bundle formation GO:0001578 ISS
    positive regulation of BMP signaling pathway GO:0030513 IMP
    astral microtubule organization GO:0030953 ISO
    astral microtubule organization GO:0030953 ISS
    positive regulation of microtubule polymerization GO:0031116 ISO
    positive regulation of microtubule polymerization GO:0031116 ISS
    positive regulation of intracellular transport GO:0032388 ISO
    positive regulation of intracellular transport GO:0032388 ISS
    positive regulation of keratinocyte differentiation GO:0045618 IMP
    cell division GO:0051301 IEA
    meiotic cell cycle GO:0051321 IEA
    positive regulation of hair follicle development GO:0051798 IMP
    positive regulation of chromosome segregation GO:0051984 ISO
    positive regulation of chromosome segregation GO:0051984 ISS
    anastral spindle assembly GO:0055048 IEA
    anastral spindle assembly GO:0055048 ISO
    regulation of mitotic spindle organization GO:0060236 ISO
    regulation of mitotic spindle organization GO:0060236 ISS
    regulation of metaphase plate congression GO:0090235 ISO
    regulation of metaphase plate congression GO:0090235 ISS
    positive regulation of protein localization to spindle pole body GO:1902365 ISO
    positive regulation of protein localization to spindle pole body GO:1902365 ISS
    positive regulation of mitotic spindle elongation GO:1902846 IEA
    positive regulation of mitotic spindle elongation GO:1902846 ISO
    positive regulation of protein localization to cell cortex GO:1904778 ISO
    positive regulation of protein localization to cell cortex GO:1904778 ISS
    positive regulation of chromosome separation GO:1905820 IEA
    positive regulation of chromosome separation GO:1905820 ISO
    positive regulation of spindle assembly GO:1905832 ISO
    positive regulation of spindle assembly GO:1905832 ISS
Subcellular Localization
    spindle pole GO:0000922 IBA
    spindle pole GO:0000922 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    chromosome GO:0005694 IEA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    spindle microtubule GO:0005876 IBA
    spindle microtubule GO:0005876 ISO
    spindle microtubule GO:0005876 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell cortex GO:0005938 ISO
    lateral plasma membrane GO:0016328 IEA
    nuclear matrix GO:0016363 IDA
    nuclear matrix GO:0016363 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    spindle pole centrosome GO:0031616 ISO
    spindle pole centrosome GO:0031616 ISS
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    microtubule plus-end GO:0035371 ISO
    microtubule plus-end GO:0035371 ISS
    microtubule minus-end GO:0036449 ISO
    microtubule minus-end GO:0036449 ISS
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    cortical microtubule GO:0055028 ISO
    cortical microtubule GO:0055028 ISS
    mitotic spindle astral microtubule GO:0061673 ISO
    mitotic spindle astral microtubule GO:0061673 ISS
    mitotic spindle GO:0072686 ISO
    microtubule bundle GO:0097427 ISO
    microtubule bundle GO:0097427 ISS
    mitotic spindle pole GO:0097431 IDA
    mitotic spindle pole GO:0097431 ISO
    lateral cell cortex GO:0097575 IMP
    cell cortex region GO:0099738 IDA
    cell cortex region GO:0099738 ISO
    cytoplasmic microtubule bundle GO:1905720 ISO
    cytoplasmic microtubule bundle GO:1905720 ISS
    mitotic spindle midzone GO:1990023 ISO
    mitotic spindle midzone GO:1990023 ISS
 Experiment description of studies that identified Numa1 in sEVs
1
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Numa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Brap  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Fmr1  
Affinity Capture-MS Mus musculus
3 Smarca2  
Affinity Capture-MS Mus musculus
4 Smarca4  
Affinity Capture-MS Mus musculus
5 Pou5f1  
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
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