Gene description for Ano1
Gene name anoctamin 1, calcium activated chloride channel
Gene symbol Ano1
Other names/aliases Tmem16a
Species Mus musculus
 Database cross references - Ano1
ExoCarta ExoCarta_101772
Entrez Gene 101772
UniProt Q8BHY3  
 Ano1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ano1
Molecular Function
    phospholipid scramblase activity GO:0017128 IDA
    intracellular calcium activated chloride channel activity GO:0005229 ISO
    protein homodimerization activity GO:0042803 IPI
    calcium activated cation channel activity GO:0005227 ISO
    protein heterodimerization activity GO:0046982 IPI
    chloride channel activity GO:0005254 IEA
Biological Process
    chloride transport GO:0006821 ISO
    transport GO:0006810 IEA
    trachea development GO:0060438 IMP
    chloride transmembrane transport GO:1902476 ISO
    regulation of membrane potential GO:0042391 IMP
    multicellular organismal development GO:0007275 IEA
    cation transport GO:0006812 ISO
    ion transport GO:0006811 IEA
    cation transmembrane transport GO:0098655 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    apical plasma membrane GO:0016324 IDA
    plasma membrane GO:0005886 ISO
    integral component of membrane GO:0016021 IEA
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IEA
    external side of plasma membrane GO:0009897 IDA
    chloride channel complex GO:0034707 IEA
 Experiment description of studies that identified Ano1 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ano1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ano1 is involved
PathwayEvidenceSource
Stimuli-sensing channels IEA Reactome





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