Gene description for CALCRL
Gene name calcitonin receptor-like
Gene symbol CALCRL
Other names/aliases CGRPR
CRLR
Species Homo sapiens
 Database cross references - CALCRL
ExoCarta ExoCarta_10203
Vesiclepedia VP_10203
Entrez Gene 10203
HGNC 16709
MIM 114190
UniProt Q16602  
 CALCRL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CALCRL
Molecular Function
    adrenomedullin receptor activity GO:0001605 IBA
    adrenomedullin receptor activity GO:0001605 IDA
    adrenomedullin receptor activity GO:0001605 IPI
    calcitonin gene-related peptide receptor activity GO:0001635 IBA
    calcitonin gene-related peptide receptor activity GO:0001635 IDA
    calcitonin gene-related peptide receptor activity GO:0001635 IPI
    G protein-coupled receptor activity GO:0004930 TAS
    calcitonin receptor activity GO:0004948 IEA
    protein binding GO:0005515 IPI
    adrenomedullin binding GO:1990409 IPI
Biological Process
    angiogenesis GO:0001525 IBA
    angiogenesis GO:0001525 IDA
    calcium ion transport GO:0006816 IDA
    cell surface receptor signaling pathway GO:0007166 IEA
    G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger GO:0007187 TAS
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IDA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IPI
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 NAS
    heart development GO:0007507 IEA
    protein transport GO:0015031 IDA
    receptor internalization GO:0031623 IDA
    cellular response to sucrose stimulus GO:0071329 IDA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IEA
    calcitonin gene-related peptide receptor signaling pathway GO:1990408 IDA
    calcitonin gene-related peptide receptor signaling pathway GO:1990408 IPI
    adrenomedullin receptor signaling pathway GO:1990410 IDA
    adrenomedullin receptor signaling pathway GO:1990410 IPI
    adrenomedullin receptor signaling pathway GO:1990410 NAS
    vascular associated smooth muscle cell proliferation GO:1990874 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IDA
    endosome GO:0005768 IDA
    endoplasmic reticulum GO:0005783 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    adrenomedullin receptor complex GO:1903143 IDA
    adrenomedullin receptor complex GO:1903143 IPI
    adrenomedullin receptor complex GO:1903143 ISS
    CGRP receptor complex GO:1990406 IDA
 Experiment description of studies that identified CALCRL in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CALCRL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CALCA  
Co-purification Homo sapiens
2 RAMP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 Ramp1  
Reconstituted Complex Rattus norvegicus
4 RAMP3  
Reconstituted Complex Homo sapiens
5 CRCP  
Affinity Capture-Western Homo sapiens
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