Gene description for Smek2
Gene name SMEK homolog 2, suppressor of mek1 (Dictyostelium)
Gene symbol Smek2
Other names/aliases AW011752
AW557776
mKIAA1387
Species Mus musculus
 Database cross references - Smek2
ExoCarta ExoCarta_104570
Vesiclepedia VP_104570
Entrez Gene 104570
UniProt Q922R5  
 Smek2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Smek2
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase regulator activity GO:0019888 IEA
    protein phosphatase activator activity GO:0072542 IBA
Biological Process
    gluconeogenesis GO:0006094 IDA
    protein dephosphorylation GO:0006470 IMP
    DNA damage response GO:0006974 IBA
    positive regulation of gluconeogenesis GO:0045722 IDA
    regulation of double-strand break repair GO:2000779 IBA
    regulation of double-strand break repair GO:2000779 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    protein phosphatase 4 complex GO:0030289 IBA
    protein phosphatase 4 complex GO:0030289 IDA
    protein phosphatase 4 complex GO:0030289 ISO
 Experiment description of studies that identified Smek2 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Smek2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Smek2 is involved
No pathways found





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