Gene description for Iars
Gene name isoleucine-tRNA synthetase
Gene symbol Iars
Other names/aliases 2510016L12Rik
AI327140
AU044614
E430001P04Rik
ILRS
Species Mus musculus
 Database cross references - Iars
ExoCarta ExoCarta_105148
Entrez Gene 105148
UniProt Q8BU30  
 Iars identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Iars
Molecular Function
    aminoacyl-tRNA editing activity GO:0002161 IEA
    nucleotide binding GO:0000166 IEA
    ligase activity GO:0016874 IEA
    isoleucine-tRNA ligase activity GO:0004822 ISO
    aminoacyl-tRNA ligase activity GO:0004812 IEA
    GTPase binding GO:0051020 ISO
    ATP binding GO:0005524 IEA
Biological Process
    tRNA aminoacylation for protein translation GO:0006418 IEA
    translation GO:0006412 IEA
    osteoblast differentiation GO:0001649 ISO
    isoleucyl-tRNA aminoacylation GO:0006428 ISO
Subcellular Localization
    cytosol GO:0005829 IBA
    membrane GO:0016020 ISO
    nucleoplasm GO:0005654 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Iars in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Iars
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Iars is involved
No pathways found





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