Gene description for Ano6
Gene name anoctamin 6
Gene symbol Ano6
Other names/aliases 2900059G15Rik
AA407480
AW554778
F730003B03Rik
Tmem16f
Species Mus musculus
 Database cross references - Ano6
ExoCarta ExoCarta_105722
Vesiclepedia VP_105722
Entrez Gene 105722
UniProt Q6P9J9  
 Ano6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ano6
Molecular Function
    calcium-activated cation channel activity GO:0005227 IDA
    calcium-activated cation channel activity GO:0005227 ISO
    intracellularly calcium-gated chloride channel activity GO:0005229 IDA
    intracellularly calcium-gated chloride channel activity GO:0005229 IDA
    intracellularly calcium-gated chloride channel activity GO:0005229 IMP
    intracellularly calcium-gated chloride channel activity GO:0005229 ISO
    voltage-gated monoatomic ion channel activity GO:0005244 IDA
    voltage-gated chloride channel activity GO:0005247 ISO
    chloride channel activity GO:0005254 IBA
    phospholipid scramblase activity GO:0017128 IDA
    phospholipid scramblase activity GO:0017128 IMP
    phospholipid scramblase activity GO:0017128 ISO
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    dendritic cell chemotaxis GO:0002407 IMP
    activation of blood coagulation via clotting cascade GO:0002543 ISO
    chloride transport GO:0006821 IDA
    chloride transport GO:0006821 IMP
    chloride transport GO:0006821 ISO
    blood coagulation GO:0007596 IMP
    plasma membrane phospholipid scrambling GO:0017121 IMP
    plasma membrane phospholipid scrambling GO:0017121 ISO
    positive regulation of bone mineralization GO:0030501 IMP
    positive regulation of bone mineralization GO:0030501 ISO
    bleb assembly GO:0032060 IMP
    bleb assembly GO:0032060 ISO
    positive regulation of monoatomic ion transmembrane transport GO:0034767 IMP
    positive regulation of monoatomic ion transmembrane transport GO:0034767 ISO
    purinergic nucleotide receptor signaling pathway GO:0035590 IMP
    purinergic nucleotide receptor signaling pathway GO:0035590 ISO
    bone mineralization involved in bone maturation GO:0035630 IMP
    sodium ion transmembrane transport GO:0035725 ISO
    positive regulation of apoptotic process GO:0043065 IMP
    phospholipid translocation GO:0045332 IDA
    negative regulation of cell volume GO:0045794 IMP
    negative regulation of cell volume GO:0045794 ISO
    pore complex assembly GO:0046931 ISO
    establishment of localization in cell GO:0051649 IDA
    establishment of localization in cell GO:0051649 IMP
    regulation of postsynaptic membrane potential GO:0060078 IDA
    regulation of postsynaptic membrane potential GO:0060078 IMP
    positive regulation of phagocytosis, engulfment GO:0060100 IMP
    calcium activated phospholipid scrambling GO:0061588 IMP
    calcium activated phosphatidylserine scrambling GO:0061589 IBA
    calcium activated phosphatidylserine scrambling GO:0061589 IDA
    calcium activated phosphatidylcholine scrambling GO:0061590 IBA
    calcium activated phosphatidylcholine scrambling GO:0061590 IDA
    calcium activated galactosylceramide scrambling GO:0061591 IDA
    calcium ion transmembrane transport GO:0070588 IGI
    calcium ion transmembrane transport GO:0070588 ISO
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 IMP
    positive regulation of monocyte chemotaxis GO:0090026 IMP
    positive regulation of monocyte chemotaxis GO:0090026 ISO
    phosphatidylserine exposure on blood platelet GO:0097045 ISO
    chloride transmembrane transport GO:1902476 IBA
    chloride transmembrane transport GO:1902476 IDA
    chloride transmembrane transport GO:1902476 IMP
    chloride transmembrane transport GO:1902476 ISO
    positive regulation of potassium ion export across plasma membrane GO:1903766 ISO
    positive regulation of potassium ion export across plasma membrane GO:1903766 ISS
    positive regulation of endothelial cell apoptotic process GO:2000353 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    chloride channel complex GO:0034707 IEA
    chloride channel complex GO:0034707 ISO
    synaptic membrane GO:0097060 IDA
    cholinergic synapse GO:0098981 IDA
    cholinergic synapse GO:0098981 IMP
 Experiment description of studies that identified Ano6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ano6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here