Gene description for Nckap1l
Gene name NCK associated protein 1 like
Gene symbol Nckap1l
Other names/aliases 4930568P13Rik
AI463083
Hem1
Hemp1
Species Mus musculus
 Database cross references - Nckap1l
ExoCarta ExoCarta_105855
Entrez Gene 105855
 Nckap1l identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Nckap1l
Molecular Function
    GTPase activator activity GO:0005096 ISO
    protein complex binding GO:0032403 ISO
    protein kinase activator activity GO:0030295 ISO
Biological Process
    positive regulation of protein kinase activity GO:0045860 ISO
    maintenance of cell polarity GO:0030011 ISO
    B cell receptor signaling pathway GO:0050853 ISO
    positive regulation of actin filament polymerization GO:0030838 ISO
    actin polymerization-dependent cell motility GO:0070358 ISO
    protein complex assembly GO:0006461 IMP
    positive regulation of cell adhesion mediated by integrin GO:0033630 IMP
    response to drug GO:0042493 ISO
    B cell homeostasis GO:0001782 IMP
    positive regulation of B cell proliferation GO:0030890 ISO
    T cell homeostasis GO:0043029 IMP
    positive regulation of gamma-delta T cell differentiation GO:0045588 IMP
    positive regulation of B cell differentiation GO:0045579 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of GTPase activity GO:0043547 ISO
    positive regulation of phosphorylation GO:0042327 ISO
    positive regulation of neutrophil chemotaxis GO:0090023 IMP
    negative regulation of interleukin-17 production GO:0032700 IMP
    neutrophil chemotaxis GO:0030593 ISO
    chemotaxis GO:0006935 ISO
    negative regulation of myosin-light-chain-phosphatase activity GO:0035509 ISO
    positive regulation of CD4-positive, alpha-beta T cell differentiation GO:0043372 IMP
    positive regulation of CD8-positive, alpha-beta T cell differentiation GO:0043378 IMP
    positive regulation of T cell proliferation GO:0042102 IMP
    myeloid cell homeostasis GO:0002262 IMP
    positive regulation of lymphocyte differentiation GO:0045621 IMP
    positive regulation of erythrocyte differentiation GO:0045648 IMP
    cortical actin cytoskeleton organization GO:0030866 ISO
    positive regulation of phagocytosis, engulfment GO:0060100 IMP
    negative regulation of interleukin-6 production GO:0032715 IMP
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
    SCAR complex GO:0031209 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Nckap1l in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Nckap1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nckap1l is involved
No pathways found





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