Gene description for Rab31
Gene name RAB31, member RAS oncogene family
Gene symbol Rab31
Other names/aliases 1700093E07Rik
AI415285
Rab22B
Species Mus musculus
 Database cross references - Rab31
ExoCarta ExoCarta_106572
Vesiclepedia VP_106572
Entrez Gene 106572
UniProt Q921E2  
 Rab31 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab31
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
Biological Process
    intracellular protein transport GO:0006886 IBA
    endocytosis GO:0006897 IBA
    receptor internalization GO:0031623 ISO
    receptor internalization GO:0031623 ISS
    cellular response to insulin stimulus GO:0032869 ISO
    cellular response to insulin stimulus GO:0032869 ISS
    Golgi to plasma membrane protein transport GO:0043001 IBA
    Golgi to plasma membrane protein transport GO:0043001 ISO
    Golgi to plasma membrane protein transport GO:0043001 ISS
    regulated exocytosis GO:0045055 ISO
    regulated exocytosis GO:0045055 ISS
    Golgi vesicle transport GO:0048193 ISO
    positive regulation of phagocytosis, engulfment GO:0060100 IMP
    phagosome maturation GO:0090382 ISO
    phagosome maturation GO:0090382 ISS
Subcellular Localization
    phagocytic cup GO:0001891 IDA
    cytoplasm GO:0005737 IDA
    early endosome GO:0005769 ISO
    early endosome GO:0005769 ISS
    late endosome GO:0005770 IEA
    late endosome GO:0005770 ISO
    trans-Golgi network GO:0005802 ISO
    endomembrane system GO:0012505 IBA
    trans-Golgi network membrane GO:0032588 IBA
    trans-Golgi network membrane GO:0032588 ISO
    trans-Golgi network membrane GO:0032588 ISS
    early phagosome membrane GO:0036186 IDA
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
 Experiment description of studies that identified Rab31 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab31
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here