Gene description for Napa
Gene name N-ethylmaleimide sensitive fusion protein attachment protein alpha
Gene symbol Napa
Other names/aliases 1500039N14Rik
AW209189
RA81
SNAPA
SNARE
a-SNAP
hyh
Species Mus musculus
 Database cross references - Napa
ExoCarta ExoCarta_108124
Vesiclepedia VP_108124
Entrez Gene 108124
UniProt Q9DB05  
 Napa identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Napa
Molecular Function
    SNARE binding GO:0000149 ISO
    SNARE binding GO:0000149 ISS
    soluble NSF attachment protein activity GO:0005483 IBA
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IBA
    syntaxin binding GO:0019905 IDA
    syntaxin binding GO:0019905 ISO
    protein-containing complex binding GO:0044877 ISO
    protein-containing complex binding GO:0044877 ISS
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IMP
    brain development GO:0007420 IMP
    regulation of synaptic vesicle priming GO:0010807 IBA
    regulation of synaptic vesicle priming GO:0010807 IDA
    regulation of synaptic vesicle priming GO:0010807 IEP
    regulation of synaptic vesicle priming GO:0010807 IGI
    regulation of synaptic vesicle priming GO:0010807 IMP
    synaptic vesicle priming GO:0016082 IDA
    synaptic vesicle priming GO:0016082 IMP
    neuron differentiation GO:0030182 IMP
    protein-containing complex disassembly GO:0032984 ISO
    synaptic transmission, glutamatergic GO:0035249 IBA
    synaptic transmission, glutamatergic GO:0035249 IGI
    SNARE complex disassembly GO:0035494 IBA
    SNARE complex disassembly GO:0035494 IGI
    apical protein localization GO:0045176 IMP
Subcellular Localization
    plasma membrane GO:0005886 IEA
    neuromuscular junction GO:0031594 ISO
    myelin sheath GO:0043209 HDA
    presynaptic active zone membrane GO:0048787 ISO
    synaptobrevin 2-SNAP-25-syntaxin-1a complex GO:0070044 IBA
    synaptobrevin 2-SNAP-25-syntaxin-1a complex GO:0070044 IDA
    synaptobrevin 2-SNAP-25-syntaxin-1a complex GO:0070044 ISO
    presynapse GO:0098793 IEA
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Napa in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Napa
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
2 Atg5  
Affinity Capture-MS Mus musculus
3 Bcl2  
Affinity Capture-MS Mus musculus
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