Gene description for Napa
Gene name N-ethylmaleimide sensitive fusion protein attachment protein alpha
Gene symbol Napa
Other names/aliases 1500039N14Rik
AW209189
RA81
SNAPA
SNARE
a-SNAP
hyh
Species Mus musculus
 Database cross references - Napa
ExoCarta ExoCarta_108124
Entrez Gene 108124
UniProt Q9DB05  
 Napa identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
 Gene ontology annotations for Napa
Molecular Function
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IDA
    soluble NSF attachment protein activity GO:0005483 IBA
    SNARE binding GO:0000149 ISO
Biological Process
    brain development GO:0007420 IMP
    neuron differentiation GO:0030182 IMP
    synaptic transmission, glutamatergic GO:0035249 IGI
    membrane fusion GO:0061025 IBA
    protein transport GO:0015031 IEA
    SNARE complex disassembly GO:0035494 IGI
    transport GO:0006810 IEA
    apical protein localization GO:0045176 IMP
    intracellular protein transport GO:0006886 IMP
    regulation of synaptic vesicle priming GO:0010807 IGI
    vesicle-mediated transport GO:0016192 IEA
Subcellular Localization
    terminal bouton GO:0043195 ISO
    intracellular GO:0005622 IMP
    vacuolar membrane GO:0005774 IBA
    synaptobrevin 2-SNAP-25-syntaxin-1a complex GO:0070044 ISO
    membrane GO:0016020 ISO
    cell GO:0005623 IMP
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 IDA
 Experiment description of studies that identified Napa in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
 Protein-protein interactions for Napa
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Napa is involved
PathwayEvidenceSource
Golgi Associated Vesicle Biogenesis IEA Reactome





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