Gene description for Slc18a1
Gene name solute carrier family 18 (vesicular monoamine), member 1
Gene symbol Slc18a1
Other names/aliases 4832416I10Rik
Vat1
Vmat1
Species Mus musculus
 Database cross references - Slc18a1
ExoCarta ExoCarta_110877
Vesiclepedia VP_110877
Entrez Gene 110877
UniProt Q8R090  
 Slc18a1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Slc18a1
Molecular Function
    serotonin:sodium:chloride symporter activity GO:0005335 IBA
    serotonin:sodium:chloride symporter activity GO:0005335 ISO
    monoamine:proton antiporter activity GO:0015311 IDA
    monoamine:proton antiporter activity GO:0015311 ISO
    enzyme binding GO:0019899 ISO
    xenobiotic transmembrane transporter activity GO:0042910 IEA
Biological Process
    aminergic neurotransmitter loading into synaptic vesicle GO:0015842 IBA
    positive regulation of dopamine secretion GO:0033603 ISO
    xenobiotic transport GO:0042908 IEA
    serotonin uptake GO:0051610 IDA
    serotonin uptake GO:0051610 ISO
    negative regulation of serotonin uptake GO:0051612 ISO
    norepinephrine uptake GO:0051620 ISO
    dopamine uptake GO:0090494 ISO
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 ISO
    secretory granule membrane GO:0030667 ISO
    synaptic vesicle membrane GO:0030672 IBA
    synaptic vesicle membrane GO:0030672 IEA
    terminal bouton GO:0043195 IBA
    synapse GO:0045202 IDA
 Experiment description of studies that identified Slc18a1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Slc18a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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