Gene description for D1Pas1
Gene name DNA segment, Chr 1, Pasteur Institute 1
Gene symbol D1Pas1
Other names/aliases Pl10
Species Mus musculus
 Database cross references - D1Pas1
ExoCarta ExoCarta_110957
Vesiclepedia VP_110957
Entrez Gene 110957
UniProt P16381  
 D1Pas1 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for D1Pas1
Molecular Function
    DNA binding GO:0003677 IEA
    DNA binding GO:0003677 ISO
    DNA helicase activity GO:0003678 ISO
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 IEA
    RNA helicase activity GO:0003724 ISO
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 ISO
    GTPase activity GO:0003924 ISO
    ATP binding GO:0005524 IEA
    transcription factor binding GO:0008134 ISO
    poly(A) binding GO:0008143 ISO
    eukaryotic initiation factor 4E binding GO:0008190 ISO
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    ribonucleoside triphosphate phosphatase activity GO:0017111 ISO
    translation initiation factor binding GO:0031369 ISO
    RNA strand annealing activity GO:0033592 ISO
    signaling adaptor activity GO:0035591 ISO
    RNA stem-loop binding GO:0035613 ISO
    gamma-tubulin binding GO:0043015 ISO
    ribosomal small subunit binding GO:0043024 ISO
    CTPase activity GO:0043273 ISO
    protein serine/threonine kinase activator activity GO:0043539 ISO
    mRNA 5'-UTR binding GO:0048027 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    pachytene GO:0000239 IMP
    chromatin remodeling GO:0006338 IEA
    translational initiation GO:0006413 ISO
    chromosome segregation GO:0007059 ISO
    gamete generation GO:0007276 IBA
    spermatogenesis GO:0007283 IMP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 ISO
    response to virus GO:0009615 ISO
    RNA secondary structure unwinding GO:0010501 ISO
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 IBA
    Wnt signaling pathway GO:0016055 ISO
    negative regulation of translation GO:0017148 ISO
    cell differentiation GO:0030154 IBA
    cell differentiation GO:0030154 IEA
    positive regulation of cell growth GO:0030307 ISO
    negative regulation of cell growth GO:0030308 ISO
    negative regulation of protein-containing complex assembly GO:0031333 ISO
    positive regulation of protein autophosphorylation GO:0031954 ISO
    positive regulation of type I interferon production GO:0032481 ISO
    positive regulation of interferon-alpha production GO:0032727 ISO
    positive regulation of interferon-beta production GO:0032728 ISO
    stress granule assembly GO:0034063 ISO
    positive regulation of toll-like receptor 7 signaling pathway GO:0034157 ISO
    positive regulation of toll-like receptor 8 signaling pathway GO:0034161 ISO
    intracellular signal transduction GO:0035556 ISO
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 ISO
    cytosolic ribosome assembly GO:0042256 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of viral genome replication GO:0045070 ISO
    innate immune response GO:0045087 ISO
    positive regulation of translation GO:0045727 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of translational initiation GO:0045948 ISO
    lipid homeostasis GO:0055088 ISO
    cellular response to arsenic-containing substance GO:0071243 ISO
    cellular response to osmotic stress GO:0071470 ISO
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    intrinsic apoptotic signaling pathway GO:0097193 ISO
    cellular response to virus GO:0098586 ISO
    chromatin looping GO:0140588 IEA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 ISO
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISO
    positive regulation of protein acetylation GO:1901985 ISO
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 ISO
    positive regulation of protein K63-linked ubiquitination GO:1902523 ISO
    protein localization to cytoplasmic stress granule GO:1903608 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    cytosol GO:0005829 ISO
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    plasma membrane GO:0005886 ISO
    cytoplasmic stress granule GO:0010494 ISO
    cytosolic small ribosomal subunit GO:0022627 ISO
    cell leading edge GO:0031252 ISO
    P granule GO:0043186 IBA
 Experiment description of studies that identified D1Pas1 in sEVs
1
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for D1Pas1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
3 Cebpa  
Affinity Capture-MS Mus musculus
4 Usp7  
Affinity Capture-MS Mus musculus
5 Fzr1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which D1Pas1 is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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