Gene description for Prdx5
Gene name peroxiredoxin 5
Gene symbol Prdx5
Other names/aliases Aoeb166
Species Rattus norvegicus
 Database cross references - Prdx5
ExoCarta ExoCarta_113898
Entrez Gene 113898
UniProt Q9R063  
 Prdx5 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Urine 20535238    
 Gene ontology annotations for Prdx5
Molecular Function
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 ISO
    peroxynitrite reductase activity GO:0072541 ISO
    RNA polymerase III regulatory region DNA binding GO:0001016 ISO
    receptor binding GO:0005102 ISO
    peroxidase activity GO:0004601 ISO
    antioxidant activity GO:0016209 ISO
    peroxiredoxin activity GO:0051920 ISO
    protein dimerization activity GO:0046983 ISO
    oxidoreductase activity GO:0016491 IEA
Biological Process
    negative regulation of apoptotic process GO:0043066 ISO
    apoptotic process GO:0006915 ISO
    regulation of apoptosis involved in tissue homeostasis GO:0060785 ISO
    response to oxidative stress GO:0006979 ISO
    hydrogen peroxide catabolic process GO:0042744 ISO
    negative regulation of transcription from RNA polymerase III promoter GO:0016480 ISO
    cellular response to reactive oxygen species GO:0034614 ISO
    oxidation-reduction process GO:0055114 IEA
    negative regulation of oxidoreductase activity GO:0051354 ISO
    reactive nitrogen species metabolic process GO:2001057 ISO
    NADPH oxidation GO:0070995 ISO
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    positive regulation of collagen biosynthetic process GO:0032967 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    mitochondrion GO:0005739 ISO
    extracellular space GO:0005615 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    nucleus GO:0005634 ISO
    peroxisomal matrix GO:0005782 ISO
    cytoplasmic vesicle GO:0031410 ISO
    peroxisome GO:0005777 ISO
 Experiment description of studies that identified Prdx5 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prdx5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prdx5 is involved
PathwayEvidenceSource
Detoxification of Reactive Oxygen Species IEA Reactome
TP53 Regulates Metabolic Genes IEA Reactome





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