Gene description for Dnm1l
Gene name dynamin 1-like
Gene symbol Dnm1l
Other names/aliases DLP1
Dnml1
Drp1
Species Rattus norvegicus
 Database cross references - Dnm1l
ExoCarta ExoCarta_114114
Entrez Gene 114114
UniProt O35303  
 Dnm1l identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Dnm1l
Molecular Function
    GTP binding GO:0005525 IEA
    identical protein binding GO:0042802 ISO
    GTPase activity GO:0003924 ISS
    ubiquitin protein ligase binding GO:0031625 ISO
    protein homodimerization activity GO:0042803 ISS
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
Biological Process
    membrane fusion GO:0061025 ISO
    positive regulation of mitochondrial fission GO:0090141 ISO
    necroptotic process GO:0070266 ISS
    regulation of ATP metabolic process GO:1903578 ISO
    protein homotetramerization GO:0051289 ISO
    mitochondrion morphogenesis GO:0070584 ISO
    peroxisome fission GO:0016559 ISS
    regulation of mitochondrion organization GO:0010821 ISO
    regulation of peroxisome organization GO:1900063 ISO
    mitochondrial fragmentation involved in apoptotic process GO:0043653 ISO
    regulation of protein oligomerization GO:0032459 ISO
    release of cytochrome c from mitochondria GO:0001836 ISO
    mitochondrial fission GO:0000266 ISS
    positive regulation of protein secretion GO:0050714 ISO
    dynamin polymerization involved in mitochondrial fission GO:0003374 ISS
    mitochondrial membrane fission GO:0090149 ISO
    positive regulation of release of cytochrome c from mitochondria GO:0090200 ISO
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
    metabolic process GO:0008152 ISS
    endocytosis GO:0006897 IEA
    regulation of mitochondrion degradation GO:1903146 ISO
    protein localization to mitochondrion GO:0070585 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    heart contraction GO:0060047 ISO
Subcellular Localization
    protein complex GO:0043234 ISO
    cytosol GO:0005829 ISS
    mitochondrion GO:0005739 ISS
    Golgi apparatus GO:0005794 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    brush border GO:0005903 ISO
    cell junction GO:0030054 IEA
    peroxisome GO:0005777 ISS
    synaptic vesicle membrane GO:0030672 IEA
    membrane GO:0016020 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    microtubule GO:0005874 ISO
    cytoplasm GO:0005737 ISO
    coated pit GO:0005905 IEA
    mitochondrial outer membrane GO:0005741 ISO
 Experiment description of studies that identified Dnm1l in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dnm1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dnm1l is involved
PathwayEvidenceSource
Apoptotic execution phase IEA Reactome





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