Gene description for Ankh
Gene name ANKH inorganic pyrophosphate transport regulator
Gene symbol Ankh
Other names/aliases Ank
Species Rattus norvegicus
 Database cross references - Ankh
ExoCarta ExoCarta_114506
Vesiclepedia VP_114506
Entrez Gene 114506
UniProt P58366  
 Ankh identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ankh
Molecular Function
    phosphate transmembrane transporter activity GO:0005315 IBA
    phosphate transmembrane transporter activity GO:0005315 IEA
    phosphate transmembrane transporter activity GO:0005315 ISO
    phosphate transmembrane transporter activity GO:0005315 TAS
    ATP transmembrane transporter activity GO:0005347 IEA
    ATP transmembrane transporter activity GO:0005347 ISO
    inorganic diphosphate transmembrane transporter activity GO:0030504 IBA
    inorganic diphosphate transmembrane transporter activity GO:0030504 IEA
    inorganic diphosphate transmembrane transporter activity GO:0030504 ISO
    inorganic diphosphate transmembrane transporter activity GO:0030504 ISS
Biological Process
    phosphate ion transport GO:0006817 TAS
    biological_process GO:0008150 ND
    gene expression GO:0010467 IEA
    gene expression GO:0010467 ISO
    bone mineralization GO:0030282 IEA
    bone mineralization GO:0030282 ISO
    regulation of bone mineralization GO:0030500 IEA
    regulation of bone mineralization GO:0030500 ISO
    regulation of bone mineralization GO:0030500 ISO
    regulation of bone mineralization GO:0030500 ISS
    regulation of bone mineralization GO:0030500 TAS
    inorganic diphosphate transport GO:0030505 IEA
    inorganic diphosphate transport GO:0030505 ISO
    phosphate ion transmembrane transport GO:0035435 IEA
    muscle cell cellular homeostasis GO:0046716 IEA
    muscle cell cellular homeostasis GO:0046716 ISO
    phosphate ion homeostasis GO:0055062 IEA
    phosphate ion homeostasis GO:0055062 ISO
    calcium ion homeostasis GO:0055074 IEA
    calcium ion homeostasis GO:0055074 ISO
    diphosphate metabolic process GO:0071344 IEA
    diphosphate metabolic process GO:0071344 ISO
    cementum mineralization GO:0071529 IEA
    cementum mineralization GO:0071529 ISO
    inhibition of non-skeletal tissue mineralization GO:0140928 IEA
    inhibition of non-skeletal tissue mineralization GO:0140928 ISO
    response to sodium phosphate GO:1904383 IEA
    response to sodium phosphate GO:1904383 ISO
    ATP export GO:1904669 IEA
    ATP export GO:1904669 ISO
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular region GO:0005576 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
 Experiment description of studies that identified Ankh in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ankh
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ankh is involved
PathwayEvidenceSource
Miscellaneous transport and binding events IEA Reactome
Transport of small molecules IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here