Gene description for Ddx39b
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
Gene symbol Ddx39b
Other names/aliases Bat1
Bat1a
p47
Species Rattus norvegicus
 Database cross references - Ddx39b
ExoCarta ExoCarta_114612
Entrez Gene 114612
UniProt Q63413  
 Ddx39b identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ddx39b
Molecular Function
    RNA-dependent ATPase activity GO:0008186 ISO
    U4 snRNA binding GO:0030621 ISO
    poly(A) RNA binding GO:0044822 ISO
    U6 snRNA binding GO:0017070 ISO
    protein complex binding GO:0032403 IDA
    ATP-dependent protein binding GO:0043008 ISO
    ATP-dependent RNA helicase activity GO:0004004 ISO
    ATP binding GO:0005524 IEA
Biological Process
    mRNA export from nucleus GO:0006406 ISO
    cellular response to DNA damage stimulus GO:0006974 IBA
    negative regulation of DNA damage checkpoint GO:2000002 ISS
    regulation of gene expression GO:0010468 IBA
    liver development GO:0001889 IEP
    mRNA splicing, via spliceosome GO:0000398 ISO
    spliceosomal complex assembly GO:0000245 ISO
    viral mRNA export from host cell nucleus GO:0046784 ISO
    RNA splicing GO:0008380 ISO
    positive regulation of DNA-templated transcription, elongation GO:0032786 ISS
    RNA secondary structure unwinding GO:0010501 ISO
Subcellular Localization
    nuclear matrix GO:0016363 IDA
    U4 snRNP GO:0005687 ISO
    nucleus GO:0005634 ISO
    nuclear speck GO:0016607 IEA
    spliceosomal complex GO:0005681 ISO
    plasma membrane GO:0005886 IDA
    U6 snRNP GO:0005688 ISO
    transcription export complex GO:0000346 ISO
    protein complex GO:0043234 IDA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Ddx39b in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ddx39b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ddx39b is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here