Gene description for Adam9
Gene name a disintegrin and metallopeptidase domain 9 (meltrin gamma)
Gene symbol Adam9
Other names/aliases AU020942
MDC9
Mltng
mKIAA0021
Species Mus musculus
 Database cross references - Adam9
ExoCarta ExoCarta_11502
Entrez Gene 11502
UniProt Q61072  
 Adam9 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Adam9
Molecular Function
    laminin binding GO:0043236 ISO
    collagen binding GO:0005518 ISO
    metalloendopeptidase activity GO:0004222 ISO
    metallopeptidase activity GO:0008237 ISO
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 ISO
    zinc ion binding GO:0008270 IEA
    integrin binding GO:0005178 ISO
    protein kinase C binding GO:0005080 IPI
    hydrolase activity GO:0016787 IEA
    peptidase activity GO:0008233 IEA
Biological Process
    positive regulation of membrane protein ectodomain proteolysis GO:0051044 IDA
    monocyte activation GO:0042117 ISO
    cell adhesion mediated by integrin GO:0033627 ISO
    cell adhesion GO:0007155 ISO
    proteolysis GO:0006508 IEA
    positive regulation of macrophage fusion GO:0034241 ISO
    response to glucocorticoid GO:0051384 IMP
    positive regulation of keratinocyte migration GO:0051549 ISO
    response to calcium ion GO:0051592 ISO
    membrane protein ectodomain proteolysis GO:0006509 ISO
    positive regulation of protein secretion GO:0050714 ISO
    cell-matrix adhesion GO:0007160 ISO
    positive regulation of cell adhesion mediated by integrin GO:0033630 ISO
    response to manganese ion GO:0010042 ISO
    response to tumor necrosis factor GO:0034612 ISO
    cellular response to lipopolysaccharide GO:0071222 ISO
    PMA-inducible membrane protein ectodomain proteolysis GO:0051088 ISO
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    response to hydrogen peroxide GO:0042542 ISO
    activation of MAPKK activity GO:0000186 ISO
Subcellular Localization
    cell surface GO:0009986 ISO
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 ISO
    intrinsic component of external side of plasma membrane GO:0031233 IDA
    basolateral plasma membrane GO:0016323 ISO
    membrane GO:0016020 IEA
    extracellular space GO:0005615 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified Adam9 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Adam9
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Adam9 is involved
PathwayEvidenceSource
Collagen degradation IEA Reactome





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