Gene description for Adh5
Gene name alcohol dehydrogenase 5 (class III), chi polypeptide
Gene symbol Adh5
Other names/aliases Adh-5
Adh3
GSNOR
Species Mus musculus
 Database cross references - Adh5
ExoCarta ExoCarta_11532
Vesiclepedia VP_11532
Entrez Gene 11532
UniProt P28474  
 Adh5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Adh5
Molecular Function
    alcohol dehydrogenase (NAD+) activity GO:0004022 IBA
    alcohol dehydrogenase (NAD+) activity GO:0004022 IDA
    alcohol dehydrogenase (NAD+) activity GO:0004022 ISO
    fatty acid binding GO:0005504 IEA
    fatty acid binding GO:0005504 ISO
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    formaldehyde dehydrogenase (NAD+) activity GO:0018467 IEA
    formaldehyde dehydrogenase (NAD+) activity GO:0018467 ISO
    identical protein binding GO:0042802 IPI
    S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity GO:0051903 IBA
    S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity GO:0051903 IMP
    S-nitrosoglutathione reductase (NADH) activity GO:0080007 IEA
    S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity GO:0106321 IEA
    S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity GO:0106322 IEA
Biological Process
    retinoid metabolic process GO:0001523 IMP
    respiratory system process GO:0003016 IMP
    ethanol catabolic process GO:0006068 ISO
    fatty acid omega-oxidation GO:0010430 ISO
    fatty acid omega-oxidation GO:0010430 ISS
    peptidyl-cysteine S-nitrosylation GO:0018119 IMP
    response to lipopolysaccharide GO:0032496 IMP
    positive regulation of blood pressure GO:0045777 IMP
    formaldehyde catabolic process GO:0046294 IBA
    formaldehyde catabolic process GO:0046294 IMP
    response to nitrosative stress GO:0051409 IMP
    response to redox state GO:0051775 IEA
    response to redox state GO:0051775 ISO
Subcellular Localization
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IBA
 Experiment description of studies that identified Adh5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Adh5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Trmt61a  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Adh5 is involved
PathwayEvidenceSource
Biological oxidations IEA Reactome
Ethanol oxidation IEA Reactome
Metabolism IEA Reactome
Phase I - Functionalization of compounds IEA Reactome





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